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Application of quetiapine fumarate in preparing medicine for treating glioma

A technology of quetiapine fumarate and glioma, which is applied in the field of anti-tumor drugs, can solve the problems of lack of safe and effective treatment methods, rapid disease course and high mortality, achieve good treatment and prognosis, and increase sensitivity , the effect of promoting differentiation

Inactive Publication Date: 2015-12-16
李成仁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Glioma is one of the common clinical malignant tumors, which has the characteristics of high recurrence rate, rapid disease progression, and high mortality. At present, there is still a lack of safe and effective treatment methods

Method used

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  • Application of quetiapine fumarate in preparing medicine for treating glioma
  • Application of quetiapine fumarate in preparing medicine for treating glioma
  • Application of quetiapine fumarate in preparing medicine for treating glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The mouse glioblastoma cell line GL261 was used to cultivate glioma stem cells by the stem cell culture method. Take 40 adult male nude mice of 6-8 weeks, subcutaneously inoculate each with 10 5 Glioma stem cells were randomly divided into control group, KUI group, TMZ group and KUI+TMZ combined group, 10 in each group. The same volume of normal saline was injected intraperitoneally in the control group in the same way; the KUI group was given intraperitoneal injection of normal saline solution of KUI at a dose of 20 mg / kg body weight, once a day for 21 consecutive days; the TMZ group was given intraperitoneal The normal saline solution was injected with TMZ at a dose of 20 mg / kg body weight, administered once every 2 days, and continued for up to 21 days; the KUI+TMZ group was given intraperitoneal injection of KUI and TMZ normal saline solution at the same time. The dosage is 20 mg / kg body weight, administered once a day for 21 consecutive days, and the dosage of TMZ...

Embodiment 2

[0057] The subcutaneous tumor in Example 1 was taken out and cut into two parts: a part was extracted with RIPA lysate and fresh 1% PMSF to extract tumor tissue protein, then Westernblotting was used to detect oligodendrocyte-associated protein (MBP) and astrocyte Expression of cytoplasmic cell markers (Vimentin, GFAP); the other part was fixed with 4% paraformaldehyde solution and then routinely embedded in paraffin and sectioned (thickness 5 μm). After blocking the tissue with bovine serum albumin solution, incubate overnight with mouse anti-PCNA, mouse anti-Nestin, rabbit anti-Olig2, goat anti-MBP, and rabbit anti-GFAP, and then incubate with corresponding biotin-labeled secondary antibody for 2 hours at room temperature , DAB color developed, mounted, observed and photographed under a microscope, and analyzed with image analysis software Image-ProPlus5.0. The result is as figure 2 shown.

[0058] figure 2 Among them, PCNA is proliferating cell nuclear antigen in gliom...

Embodiment 3

[0063] Take 40 8-week-old adult male C57 mice and inoculate each mouse with 10 microinjectors under the brain positioning system 4 Glioma stem cells were randomly divided into control group, KUI group, TMZ group and KUI+TMZ combined group, 10 in each group. The same volume of normal saline was injected intraperitoneally in the control group in the same way; the KUI group was given intraperitoneal injection of KUI normal saline solution at a dose of 20 mg / kg body weight, once a day for 21 consecutive days; the TMZ group was given intraperitoneal injection TMZ normal saline solution treatment, the dose is 20mg / kg body weight, administered once every 2 days, continuous administration up to 21 days; KUI+TMZ group was given intraperitoneal injection of KUI and TMZ normal saline solution treatment at the same time, the dose of KUI The dose of TMZ is 20 mg / kg body weight, administered once a day for 21 consecutive days, and the dose of TMZ is 20 mg / kg body weight, administered once e...

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Abstract

The invention relates to the field of antitumor drugs, and in particular relates to an application of quetiapine fumarate in preparing a medicine for treating glioma. Through in-vivo and in-vitro experiments, the effects of resisting glioma and glioma stem cells of quetiapine fumarate (KUI) and the effects obtained by mixing KUI with chemotherapy drug temozolomide (TMZ) are investigated; quetiapine fumarate can inhibit growth of glioma, and promote the glioma stem cells to express markers of oligodendroglia cells, and after being mixed with TMZ for application, quetiapine fumarate increases the sensitivity of the glioma cells for temozolomide, and the inbibiting effect is better; after temozolomide acts, quetiapine fumarate can delay the growth of recurrent glioma; quetiapine fumarate can promote the glioma stem cells to differentiate and mature towards the oligodendroglia like cells by taking the glioma stem cells as the action target. Therefore, quetiapine fumarate can be used for preparing the medicine for treating glioma, and achieves good effects for treating and prognosis of glioma.

Description

technical field [0001] The invention relates to the field of antitumor drugs, in particular to the application of quetiapine fumarate in the preparation of drugs for treating glioma. Background technique [0002] Glioma is one of the common clinical malignant tumors, which has the characteristics of high recurrence rate, rapid disease progression, and high mortality. Currently, there is still a lack of safe and effective treatment methods. Recent studies have found that the high recurrence rate of glioma may be closely related to glioma stem cells, which exist in glioma tissues and are insensitive to radiotherapy and chemotherapy, and are the root cause of glioma recurrence. Therefore, the development of therapeutic drugs targeting glioma stem cells may improve the cure rate of glioma. [0003] In view of this, the present invention is proposed. Contents of the invention [0004] The object of the present invention is to provide the application of quetiapine fumarate in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/554A61P35/00
Inventor 李成仁
Owner 李成仁
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