Rat unilateral pulmonary fibrosis model building method

A pulmonary fibrosis and rat technology, applied in the medical field, can solve the problems of huge impact on the research and development of pulmonary fibrosis treatment drugs, inconvenient research on pulmonary fibrosis diseases, uneven distribution of lesions, etc., and achieves small variation, stable results, and widespread promotion. Effect

Inactive Publication Date: 2015-12-16
NANJING KCI BIOTECH INC
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Problems solved by technology

The model has uncertain factors such as uneven distribution of lesions, large variability, and high mortality, which brings inconvenience to the research of pulmonary fibrosis diseases, especially for the development of pulmonary fibrosis treatment drugs.

Method used

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  • Rat unilateral pulmonary fibrosis model building method
  • Rat unilateral pulmonary fibrosis model building method
  • Rat unilateral pulmonary fibrosis model building method

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Embodiment Construction

[0010] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0011] Taking Sprgue-Dawley (SD) male rats as an example, a method for making an animal model of unilateral lung pulmonary fibrosis is provided, comprising the steps of:

[0012] (1) Animals were induced and anesthetized with 50 mg / kg pentobarbital sodium.

[0013] (2) The midline of the animal's neck was shaved, disinfected, and the main trachea was exposed by incision. A tracheotomy was performed between the cartilage rings of the trachea, the trachea was intubated, and a ventilator was connected. The breathing ra...

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Abstract

The invention relates to the technical field of medical treatment and especially relates to a method for making a rat unilateral pulmonary fibrosis model. The method comprises animal anesthetization, tracheal intubation, bleomycin perfusion into unilateral lung, and postoperative care, wherein concentrations of bleomycin for perfusion into unilateral lung is 1.5, 3.0 and 5.0mg / kg. The method has the advantages of simple making processes, wide popularization range, high experiment setting ratio, stable result, small pathology variation and low mortality. The rat unilateral pulmonary fibrosis model has a pathological process high similar to a human chronic pulmonary fibrosis pathological process and thus the rat unilateral pulmonary fibrosis model has a high etiology and pathology research value and has an important meaning for evaluation of pulmonary fibrosis-resistant drugs.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for making an animal model of experimental rat unilateral pulmonary fibrosis. Background technique [0002] Chronic pulmonary fibrosis is one of the major lung diseases currently causing fatal lung diseases worldwide. A rapid, efficient, and low-mortality rodent model of pulmonary fibrosis is required for current studies of the etiology, pathology, and pharmacology of this disease. Currently used bleomycin-induced pulmonary fibrosis models in rodents are all non-selective bilateral lung fibrosis. The model has its own uncertain factors such as uneven distribution of lesions, large variability, and high mortality, which brings inconvenience to the research of pulmonary fibrosis diseases, especially has a great impact on the development of pulmonary fibrosis treatment drugs. Therefore, our team established a model of unilateral pulmonary fibrosis in rats, which made up ...

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Application Information

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IPC IPC(8): A61K38/14
CPCA61K38/14A61K49/0008
Inventor 庄永杰陈继巧
Owner NANJING KCI BIOTECH INC
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