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Node order application method of high concentration IAA and glycerin gel

A glycerin gel, high-concentration technology, applied in the field of plant biology, can solve problems such as high cost, loss of branches, death, etc., and achieve the effect of easy operation and reduced water evaporation

Inactive Publication Date: 2015-12-23
钱夕刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] A method for using high-concentration IAA and glycerin gel at nodes, characterized in that the method includes preparation of hormone mother solution, preparation of working solution, preparation of hormone gel, cutting, dripping at axillary buds, and the specific operation steps are as follows:

[0014] ① Hormone mother solution preparation: Accurately weigh 0.1g of IAA and dissolve it in a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL IAA mother solution;

[0015] ②Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:2 into a high-concentration hormone working solution with a concentration of 500mg / L, and add 10ml of glycerin to 90ml of hormone working solution at the same time;

[0016] ③ Hormone gel preparation: Accurately weigh 1g of sodium carboxymethylcellulose and 8g of sodium alginate, and dissolve them in 100mL of hormone and glycerin working solution with a concentration of 500mg / ...

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PUM

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Abstract

The invention discloses a node order application method of high concentration IAA and glycerin gel, and belongs to the technical field of plant biology. The application method comprises the following steps: at first, preparing a IAA mother liquor with a concentration of 1 mg / mL, diluting the mother liquor into a high-concentration IAA work fluid with a concentration of 500 mg / L; using the work fluid to prepare a high-concentration IAA and glycerin gel confining liquid, which contains sodium carboxymethyl cellulose with a mass concentration of 1% and sodium alginate with a mass concentration of 8%; inserting branches into cultivation matrix; and dropwise adding the gel confining liquid on axillary buds of healthy and strong branches.

Description

technical field [0001] The invention discloses a method for using high-concentration IAA and glycerin gel at nodal sites, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/38A01P21/00A01G1/00A01G7/06
Inventor 钱夕刚
Owner 钱夕刚