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Determination method for fractional value of stable carbon isotopes in carbon dioxide assimilation process of microalgae

A technology for stabilizing carbon isotopes and carbon dioxide, applied in material separation, analyzing materials, measuring devices, etc., can solve problems such as inability to determine the fractionation value of stable carbon isotopes in vivo, and achieve convenient carbon sink research, high accuracy, and reliable data Effect

Active Publication Date: 2015-12-23
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for determining the stable carbon isotope fractionation value in the microalgal carbon dioxide assimilation process, so as to overcome the inability to determine the stable carbon in vivo by designing a multi-step in vitro reaction process and step analysis in the prior art. Insufficient isotope fractionation value

Method used

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  • Determination method for fractional value of stable carbon isotopes in carbon dioxide assimilation process of microalgae
  • Determination method for fractional value of stable carbon isotopes in carbon dioxide assimilation process of microalgae
  • Determination method for fractional value of stable carbon isotopes in carbon dioxide assimilation process of microalgae

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Embodiment 1

[0040] Embodiment 1: the first step, select two kinds of δ 13 Sodium bicarbonate with a C value difference greater than 10‰ was added to the detection culture solution as isotope label 1 and isotope label 2 respectively, and the microalgae to be tested with the same initial biomass were cultivated under the same culture conditions at the same time; the detection culture solution was added The concentration of sodium bicarbonate is 16mM, add 10mM acetazolamide also is the algal culture fluid of AZ simultaneously; Measure the initial biomass M of microalgae to be tested O and the initial stable carbon isotopic composition of microalgae δ 13 The value of C δ O . δ of isotope-labeled sodium bicarbonate 1 13 C value is δ C1 , the δ of isotope-labeled sodium bicarbonate 2 13 C value is δ C2 ;

[0041] The second step is to measure the final biomass M of the microalgae to be tested after being cultivated in the detection culture solution with isotope labeling for 4 days. N an...

Embodiment 2

[0055] Example 2: The detection culture medium is an algae culture medium in which the concentration of sodium bicarbonate is 20 mM and 10 mM acetazolamide, namely AZ, is added at the same time, and the others are the same as in Example 1.

[0056] The culture material is: mixed microalgae extracted from Hongfeng Lake; the culture medium is 20mM sodium bicarbonate and 10mMAZ added to the basic culture medium (SE medium), and the basic culture conditions are: photoperiod L / D: 12h / 12h; temperature 25℃; light intensity is 100μmol m -2 the s -1 , pH 8.2; δ of added sodium bicarbonate 13 C are -17.4‰ (PDB) (δ C1 ) and -28.4‰(PDB) (δ C2 ), Table 3 shows the stable carbon isotope fractionation values ​​of mixed microalgae in the process of carbon dioxide assimilation in Hongfeng Lake .

[0057] Table 3 Fractionation values ​​of stable carbon isotopes during the process of carbon dioxide assimilation by mixed microalgae in Hongfeng Lake

[0058]

[0059] It can be seen...

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Abstract

The invention discloses a determination method for the fractional value of stable carbon isotopes in the carbon dioxide assimilation process of microalgae. The determination method comprises the following steps: respectively adding two types of sodium bicarbonate with large delta<13>C value difference into a detection culture solution added with 10mM acetazolamide to culture the microalgae to be detected; obtaining a composition of the stable carbon isotopes of a newly born algae body of detected microalgae cultured in the detection culture solution labelled by two types of isotopes and the portion of added inorganic carbon source utilized by the microalgae to be detected; according to the result and the composition of the average stable carbon isotopes of atmospheric carbon dioxide in the culture environment during the culture period, obtaining the fractional value of the stable carbon isotopes in the carbon dioxide assimilation process of the microalgae. The determination method disclosed by the invention has the advantages that the fractional value of the stable carbon isotopes can be determined in vivo, and not only can the fractional value of the stable carbon isotopes of a single microalgae seed be detected, but also the fractional value of the stable carbon isotopes of mixed microalgaes can be detected, and the detected data are reliable and the accuracy of a result is high.

Description

technical field [0001] The invention relates to a method for determining the stable carbon isotope fractionation value in the microalgae carbon dioxide assimilation process, belonging to the fields of ecological environment management and marine bioengineering. Background technique [0002] Microalgae (microalgae) include all tiny plants that live in water and engage in a planktonic lifestyle, usually referring to planktonic algae. The structure of microalgae is simple, and its physiological process is also relatively simple. Some species are model plants for scientific research, such as Chlamydomonas reinhardtii and Chlorella, and many species can also be cultivated artificially, which provides convenience for our research. There are two ways for microalgae to utilize inorganic carbon in water bodies. (1) Utilize carbon dioxide in the atmosphere. CO 2 As a linear non-polar molecule, it is electrically neutral, and it can freely diffuse into the cell bilayer lipid membrane...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/00
Inventor 吴沿友谢腾祥李海涛赵丽华张开艳杭红涛王瑞姚凯饶森陆叶
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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