57 results about "Isotope fractionation" patented technology

The invention discloses a method for separating water in an alcoholic beverage for assaying an isotope of H and O. An assaying device is built through an N2 gas bottle, a bubble bottle, an absorption pipe, a condensation bottle, a three-way valve and a liquid nitrogen tank, the water in the alcoholic beverage is effectively separated by using an evaporation-capturing-liquid nitrogen condensation technology, and the isotope ratio of the H and the O is assayed through an elemental analysis-stable isotope mass spectrometer. According to the method, the water in various alcoholic beverages can be extracted, and the isotopes of the H and the O of the water are assayed; a sample is completely evaporated and separated, so that isotope fractionation cannot be generated in the process; a drying agent is made of a material which specifically absorbs the water and does not absorb alcohol, and impurity interference cannot be caused after separation. A low-price glass or stainless steel assembly is adopted in the process and can be recycled, so that the cost is saved. The high-accuracy testing capacity can be provided by offline separation assaying.

The invention discloses a measurement method of an inorganic nitrogen isotope fractionation value of an ammonium salt assimilated by a plant. Clonal tissue culture seedlings are cultured in culture media, in which two kinds of nitrate with different stable nitrogen isotope values serve as sole nitrogen sources, respectively; after the tissue culture seedlings are cultured for 5 weeks, the stable nitrogen isotope values of leaves of the tissue culture seedlings cultured under the two kinds of nitrate are measured; similarly, clonal tissue culture seedlings are additionally taken, and are cultured in culture media with mixed nitrogen sources formed by the ammonium salt and the two kinds of nitrate whose stable nitrogen isotope values are different respectively; the stable nitrogen isotope values of the leaves of the tissue culture seedlings cultured under the two mixed nitrogen sources are measured; shares, which are utilized by the tissue culture seedlings, of the nitrate are calculated, and accordingly, afterwards, the stable nitrogen isotope values after the ammonium salts assimilated by the tissue culture seedlings cultured under the mixed nitrogen sources generate nitrogen isotope fractionation are calculated; finally, the stable nitrogen isotope fractionation values generated by the ammonium salts assimilated by the tissue culture seedlings, namely, to-be-measured stable nitrogen isotope fractionation values generated by the ammonium salts assimilated by the plants, are obtained. The measurement method can measure the inorganic nitrogen isotope fractionation value of the ammonium salt assimilated by a plant which cannot grow due to monoammonium or a plant which grows under the mixed nitrogen source; as a culture experiment is developed by utilizing the clonal tissue culture seedlings of the same genotype, the measurement result is more reliable.

The invention discloses a lake replenishment water source tracing method using hydrogen and oxygen isotopes. The method mainly comprises the following steps of: collecting a lake water sample; measuring the abundance of the hydrogen and oxygen isotopes (2H, 18O) of the lake water; obtaining the air temperature, relative humidity, daily precipitation and evaporation data of a region where a lake islocated; constructing the atmospheric precipitation line of the research region; calculating the theoretical value of the abundance of the hydrogen and oxygen isotope under an evaporation balance andlimit state at each moment; constructing a lake dynamic evaporation process line; reducing the abundance of the hydrogen and oxygen isotopes of the water quantity of the lake under the condition thatthe water quantity of the lake is not subjected to an evaporation isotope fractionation stage; calculating the proportion of the lake evaporation capacity in inflow in each time period; using a lakewater quantity and isotope mass conservation equation to finely segment the water quantity and isotope abundance of a lake replenishment water source and consumption evaporation items in each time period so as to obtain a lake inflow water quantity Ri and a corresponding isotope abundance change-along-with-time process line; and comprehensively analyzing the change condition of the lake inflow water source.

The invention discloses a method for obtaining the total assimilative capacity of plant inorganic carbon under an outdoor ecological environment, and belongs to the fields of a crop information detecting technology, a plant physiological information detecting technology and ecological environment management. The method is characterized by calculating the share of actually assimilated carbon dioxide of plant leaves according to a stable carbon isotope fractionation value of completely assimilated carbon dioxide of the plant leaves and the stable carbon isotope fractionation value of the actually assimilated carbon dioxide of the plant leaves; converting the total assimilative capacity of the plant inorganic carbon according to actual carbon dioxide assimilative capacity measured through a portable photosynthesis system. According to the method disclosed by the invention, the carbon dioxide utilization shares of plants in different seedling ages and different growth periods under the outdoor ecological environment can be quickly, conveniently and accurately obtained in real time, and meanwhile, the bicarbonate utilization capacity of the plants under the outdoor ecological environment can also be obtained.

The invention relates to methods for determining the source of hydrocarbons presented in the pores of a host rock or found in contaminated groundwater. The method includes the steps of (a) determining a first isotopic composition of boron and / or lithium in one or more components within a potential source rock sample, such as kerogen, clay, or water; (b) determining a second isotopic composition of boron and / or lithium in the hydrocarbons found within the pores of a host rock sample or in contaminated groundwater; and (c) comparing the first and second isotopic compositions to determine whether the potential source rock is the source of the hydrocarbons within the pores of the host rock or in contaminated groundwater. The comparison is facilitated by using the isotope fractionation between the kerogen, clay, or water components and the bitumen component of the potential source rock, which allows one to predict the isotope composition of any hydrocarbons originating in the bitumen component of the source rock, based on the isotope composition of one of the other three phases. The method can be used to select host rock for extracting oil and other hydrocarbons, as well as in remediating groundwater contamination.

The invention discloses a determining method of tissue cultured seedling sucrose. The method comprises the following steps of respectively adopting beet sucrose and sugarcane sucrose as organic carbon sources to prepare a beet sucrose culture medium and a sugarcane sucrose culture medium; culturing tissue cultured seedlings with consistent growth vigors until the fresh weight is increased by over 20 times, and finishing the culturing; determining delta<13>C values of the beet sucrose, the sugarcane sucrose and newly-growing leaves; according to the tissue cultured seedlings of plants to be determined, utilizing the sugarcane sucrose and the beet sucrose to produce carbon isotope fractionation values, and utilizing a fractionation value of carbon dioxide in air, the weight increment of the tissue cultured seedlings and sugarcane consumption to calculate the sugarcane utilization rate of the tissue cultured seedlings. The method provided by the invention not only can be used for quantitatively determining the autotrophy portion, the heterotrophy portion and the like of the tissue cultured seedlings in one growth period, but also can be used for calculating the sugarcane utilization rate of the tissue cultured seedlings in the same period, has an important guide value on regulation of tissue cultured environment factors, and provides technical parameters for domestication and transplantation of later tissue cultured seedlings.