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Method for extracting cordycepin and cordyceps polysaccharide from cordyceps militaris solid medium residue

A technology of solid medium and cordyceps polysaccharides, which is applied in the field of extraction of cordycepin and cordyceps polysaccharides, can solve problems such as environmental impact and waste of resources, and achieve the effects of convenient operation, high crude extraction rate, and good social benefits

Active Publication Date: 2018-04-13
HUNAN YANDI BIOLOGICAL ENG
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Problems solved by technology

[0003] At present, the solid culture method has been widely used in the cultivation of Cordyceps militaris. The solid culture medium of Cordyceps militaris is rich in mycelia, but because there is no mature technology to extract and separate the active ingredients, the solid medium is currently used after the cultivation. Discarding as waste not only causes a waste of resources, but also has a certain impact on the environment

Method used

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Embodiment 1

[0020] Example 1 The method for extracting cordycepin and cordyceps polysaccharide from the solid medium residue of Cordyceps militaris

[0021] The method comprises the steps of drying, pulverizing, water extracting, microfiltration, concentrating, freeze-drying, qualitative and quantitative detection and the like on the residue of the solid culture medium of the Cordyceps militaris.

[0022] 1. Drying: The solid medium residue of Cordyceps militaris is dried in a hot air circulation drying oven at a temperature of ≤80°C for 2 hours.

[0023] 2. Pulverization: The dried materials are pulverized separately with a universal pulverizer, and passed through a 120-mesh sieve.

[0024] 3. Water extraction: add water to the pulverized culture medium residue and carry out hot reflux extraction at 65°C for 2 times, 2 hours each time, and the ratio of extraction material to water is 1:15 in g:mL each time.

[0025] 4. Microfiltration: Centrifuge the extract at 6000rpm for 30min after c...

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Abstract

The invention provides a method for extracting cordycepin and cordyceps polysaccharide from the residue of Cordyceps militaris solid medium, comprising drying, crushing, water extraction, microfiltration, concentration, freeze-drying and qualitative and quantitative analysis of the residue of Cordyceps militaris solid medium detection steps. The present invention uses the solid medium residue of Cordyceps militaris as raw material, adopts methods such as orthogonal test design to analyze various process conditions for extracting Cordyceps polysaccharide and cordycepin from the solid medium residue of Cordyceps militaris, and determines the best extraction method. The method of the invention has high crude improvement rate, convenient operation, can maximize the utilization of the medium residue of Cordyceps militaris, is beneficial to industrial production, and can bring good social, economic and environmental benefits.

Description

technical field [0001] The invention relates to a process for extracting cordycepin and cordyceps polysaccharide, in particular to a method for extracting cordycepin and cordyceps polysaccharide from the solid medium residue of Cordyceps militaris. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris and Cordyceps militaris, is a valuable medicinal fungus in my country. The fruiting body of Cordyceps militaris is the main edible and medicinal part of Cordyceps militaris. [0003] At present, the solid culture method has been widely used in the cultivation of Cordyceps militaris. The solid culture medium of Cordyceps militaris is rich in mycelia, but because there is no mature technology to extract and separate the active ingredients, the solid medium is currently used after the cultivation. Discarding it as waste not only causes a waste of resources, but also has a certain impact on the environment. [0004] With the acc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/16C07H1/08C08B37/00
Inventor 王玉兰刘艳赵明王春芳
Owner HUNAN YANDI BIOLOGICAL ENG
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