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Method for extracting cordycepin and cordyceps from cordyceps militaris solid medium residues

A technology of solid medium and cordyceps polysaccharides, which is applied in the field of extraction of cordycepin and cordyceps polysaccharides, can solve problems such as environmental impact and waste of resources, and achieve the effects of convenient operation, high crude extraction rate, and good social benefits

Active Publication Date: 2016-02-03
HUNAN YANDI BIOLOGICAL ENG
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Problems solved by technology

[0003] At present, the solid culture method has been widely used in the cultivation of Cordyceps militaris. The solid culture medium of Cordyceps militaris is rich in mycelia, but because there is no mature technology to extract and separate the active ingredients, the solid medium is currently used after the cultivation. Discarding as waste not only causes a waste of resources, but also has a certain impact on the environment

Method used

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Examples

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Embodiment 1

[0020] Example 1 The method for extracting cordycepin and cordyceps polysaccharide from the solid medium residue of Cordyceps militaris

[0021] The method comprises the steps of drying, pulverizing, water extracting, microfiltration, concentrating, freeze-drying, qualitative and quantitative detection and the like on the residue of the solid culture medium of the Cordyceps militaris.

[0022] 1. Drying: The solid medium residue of Cordyceps militaris is dried in a hot air circulation drying oven at a temperature of ≤80°C for 2 hours.

[0023] 2. Pulverization: The dried materials are pulverized separately with a universal pulverizer, and passed through a 120-mesh sieve.

[0024] 3. Water extraction: add water to the pulverized culture medium residue and carry out hot reflux extraction at 65°C for 2 times, 2 hours each time, and the ratio of extraction material to water is 1:15 in g:mL each time.

[0025] 4. Microfiltration: Centrifuge the extract at 6000rpm for 30min after c...

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Abstract

The invention provides a method for extracting cordycepin and cordyceps from cordyceps militaris solid medium residues. The method comprises the steps of drying, crushing, water extraction, microfiltration, condensation, freeze-drying and qualitative / quantitative detection on the cordyceps militaris solid medium residues. The method takes the cordyceps militaris solid medium residues as a raw material, employs an orthogonal experimental design method for analyzing various technical conditions for extracting the cordyceps and the cordycepin by using the cordyceps militaris solid medium residues, and determines an optimum extraction method. The method has the advantages of high crude extraction rate and convenient operation, can use the cordyceps militaris solid medium residues with a maximum limit, is benefit for industrial production, and can bring good social benefit, economic benefit and environment benefit.

Description

technical field [0001] The invention relates to a process for extracting cordycepin and cordyceps polysaccharide, in particular to a method for extracting cordycepin and cordyceps polysaccharide from the solid medium residue of Cordyceps militaris. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, is a valuable medicinal fungus in my country, and the fruiting body of Cordyceps militaris is the main edible and medicinal part of Cordyceps militaris. [0003] At present, the solid culture method has been widely used in the cultivation of Cordyceps militaris. The solid culture medium of Cordyceps militaris is rich in mycelia, but because there is no mature technology to extract and separate the active ingredients, the solid medium is currently used after the cultivation. Discarding it as waste not only causes a waste of resources, but also has a certain impact on the environment. [0004] With the acceleration of the pac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/16C07H1/08C08B37/00
Inventor 王玉兰刘艳赵明王春芳
Owner HUNAN YANDI BIOLOGICAL ENG
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