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Urine formed element candidate reference measuring method based on quantitative counting method principle

A technology of reference measurement and counting method, applied in the direction of measuring devices, analysis materials, instruments, etc., to achieve the effect of ensuring accuracy, low uncertainty and high precision

Inactive Publication Date: 2016-02-24
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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  • Application Information

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Problems solved by technology

In 2009, my country’s Blood and Body Fluids Expert Committee also put forward the “Suggestions on the Standardization of Urine Formed Components Testing”, but so far no reference method for urine formed components has been established. In order to meet the needs of urine formed components testing standardization, how to achieve Intercommunication and mutual recognition of urine test results has become an urgent technical problem to be solved

Method used

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  • Urine formed element candidate reference measuring method based on quantitative counting method principle
  • Urine formed element candidate reference measuring method based on quantitative counting method principle
  • Urine formed element candidate reference measuring method based on quantitative counting method principle

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Embodiment

[0026]Three samples of WBC and EC were respectively counted in the three groups, and the mean values ​​of WBC and EC were 217-285, 51-85; SD were 0.7-3.4, 1-1; CV were 0.3-1.1, 1.9-1.9, respectively. The results are shown in Table 1.

[0027] Table 1 Differences between counts in each group

[0028]

[0029] Chaoyang Hospital, Ditan Hospital, You'an Hospital, Fuxing Hospital, and Fangshan Hospital used the reference measurement method of the present invention to count urine white blood cells, red blood cells, and epithelial cells, and counted urine with quantitative counting boards in five hospitals. The coefficient of variation of the score is shown in Table 2.

[0030] Table 2 Coefficient of variation of urine formed components counted by quantitative counting board in five hospitals

[0031]

[0032] Taking Chaoyang Hospital as an example, the mean value, coefficient of variation and bias between the two methods were calculated for the WBC, RBC and EC reference meas...

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Abstract

The invention relates to a urine formed element candidate reference measuring method based on a quantitative counting method principle. The method comprises the steps that an instrument is selected; urine samples are collected; the urine samples are divided into three sets for counting, and six specimens are obtained for counting; all the urine sample sets are directly detected without being processed through centrifugation; all the urine sample sets are filled in counting pools of disposable counting boards through a calibrated pipettor; standing is performed for 3 minutes after the counting pools of the counting boards are filled with all the urine sample sets, and full morphology and distribution of formed elements in the counting pools are observed by adopting a low power lens of a microscope; by adopting a high power lens of the microscope, the cell constituents are observed and identified, and the number is calculated; the variable coefficient CV of epithelial cells (EC) is smaller than 14%; the variable coefficient CV of red blood cells (RBC) and white blood cells (WBC) is smaller than 7%; the standard uncertainty is synthesized according to the expanded uncertainty of counting of the urine red blood cells, the white blood cells and the epithelial cells. According to the method, the accuracy and reliability of inspection results are guaranteed, and then mutual communication and recognition of the urine inspection results can be promoted.

Description

technical field [0001] The present invention relates to a standardized method for testing urine formed components, in particular to a urine formed component (red blood cell RBC) based on the principle of quantitative counting method for calibrating and evaluating routine microscopic urine formed component analyzers. , white blood cell WBC and epithelial cell EC) candidate reference measurement methods. Background technique [0002] At present, the formed elements of urine refer to the tangible objects excreted through urine that can be observed under an optical microscope, including various cells, casts, crystals, and analysis of various pathological components, which are useful for the diagnosis, treatment, and monitoring of urinary system diseases It has important application value and is an indispensable means of examination in urinalysis. There are many methods for examining the formed components of urine, which can be divided into manual microscopic examination, urine ...

Claims

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Application Information

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IPC IPC(8): G01N33/493
CPCG01N33/493
Inventor 马怀安王清涛张瑞高志琪苑惠敏窦会东焦炳欣郭鸿雁王芳刘一君郝莹
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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