Sjogren syndrome specific autoantibody immunoblotting kit

A technology for Sjögren's syndrome and autoantibodies, applied in measurement devices, instruments, disease diagnosis, etc., can solve the problems of waste of clinical resources, no detection of immunoblot strips, and lack of disease specificity.

Inactive Publication Date: 2016-02-24
厦门敖依生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most western blot strips cover autoantibodies related to various autoimmune diseases such as systemic lupus erythematosus, scleroderma, dermatomyositis/polymyositis, lack of disease specificity, and cause waste of clini

Method used

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Examples

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Effect test

Embodiment 1

[0009] The components of this kit include: 5 plates of blotted membrane strips placed in the reaction tank, 8 strips per plate, 0.5ml of enzyme-linked reagent, 50ml of chromogenic reagent A, 50ml of chromogenic reagent B, 50ml of stop solution, 50ml of concentrated washing solution, standard zone . Sample requirements: 2-5ml of venous blood, put in a test tube and centrifuge to separate serum after coagulation, store serum samples at 2-8°C, and store samples that cannot be tested within 24 hours at -20°C, severe hemolysis, floc or mold Altered specimens can affect testing.

[0010] Measurement steps

[0011] 1) Preparation of washing working solution

[0012] Dilute the 20X concentrated washing solution stored at 2-8°C to 1000ml with distilled water or deionized water, put it into a reagent bottle, indicate the preparation time on the label, and store it at 4°C;

[0013] 2) Add 1ml sample diluent to the reaction tank containing the blotted membrane strip to wet the membrane...

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Abstract

The invention discloses an immunoblotting kit for sjogren syndrome specific autoantibody detection. The kit includes: a blotting membrane strip placed in a reaction tank, an enzyme conjugate, a chromogenic reagent A, a chromogenic reagent B, a stop solution, a dilution solution, concentrated washing liquid, and a standard zone tape. The membrane strip is placed into the reaction tank to react with to-be-tested serum, if the to-be-tested serum contains a corresponding autoantibody, the autoantibody can combine with a corresponding antigen, then the enzyme conjugate and the chromogenic reagents are added, contrast with the standard zone tape is carried out to judge whether the serum contains the sjogren syndrome specific autoantibody: anti-SSA antibody (Ro), SSB (La), salivary gland protein 1 (SP1), carbonic anhydrase 6 (CA6), and parotid secretory protein (PSP). The existence of sjogren syndrome specific autoantibodies and the antibody species and quantity are of important clinical significance for sjogren syndrome diagnosis and differential diagnosis.

Description

technical field [0001] The invention relates to a Sjogren's syndrome specific autoantibody immunoblotting kit. Background technique [0002] Sjögren's syndrome is a common autoimmune disease that mainly involves salivary glands, lacrimal glands, and many other vital organs. Autoantibodies are important biomarkers in the diagnosis of Sjögren's syndrome. At present, the autoantibodies mainly used in the diagnosis of Sjögren's syndrome are SSA and SSB. However, due to the lack of high sensitivity of SSA and SSB, the diagnosis of a large number of patients with Sjögren's syndrome (especially early patients) still requires an invasive labial gland biopsy, which brings great benefits to the diagnosis and treatment of Sjogren's syndrome. obstacle. [0003] Currently, there is no western blot kit specifically for the detection of Sjogren's syndrome-specific autoantibodies. Most western blot strips cover autoantibodies related to various autoimmune diseases such as systemic lupus ...

Claims

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Application Information

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IPC IPC(8): G01N33/564
CPCG01N33/564G01N2800/101
Inventor 不公告发明人
Owner 厦门敖依生物科技有限公司
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