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A preparation method of rgd-m13 phage/oxidized regenerated cellulose composite hemostatic material

A RGD-M13, regenerated cellulose technology, applied in pharmaceutical formulations, pharmaceutical sciences, bandages, etc., can solve the problems of reduced mechanical strength and bioabsorbable properties of oxidized regenerated cellulose, and a small increase in hemostasis time, to ensure biological Absorbable properties, improving hemostatic properties, and improving the effect of grafting rate

Active Publication Date: 2018-07-06
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the hemostatic time of the existing hemostatic material modified by oxidized regenerated cellulose is small, and the mechanical strength and bioabsorbability of oxidized regenerated cellulose are reduced, and a RGD-M13 bacteriophage is provided Preparation method of oxidized regenerated cellulose composite hemostatic material

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0022] Specific Embodiment 1: This embodiment is a preparation method of RGD-M13 bacteriophage / oxidized regenerated cellulose composite hemostatic material, which is completed according to the following steps:

[0023] 1. Activation of oxidized regenerated cellulose: immerse oxidized regenerated cellulose into 2-morpholineethanesulfonic acid with a molar concentration of 0.1mol / L, and then ultrasonically treat it for 10min~30min at an ultrasonic power of 160W~400W, then add 1-Ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide, then react at room temperature and at a stirring speed of 200r / min~550r / min for 15min ~20min to obtain activated oxidized regenerated cellulose;

[0024] The mass fraction of carboxyl groups in the oxidized regenerated cellulose in step 1 is 16% to 24%; the oxidized regenerated cellulose exists in the form of short filaments, filaments or fabrics;

[0025] The volume ratio of the amount of carboxyl in the oxidized regenera...

specific Embodiment approach 2

[0037] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the mass fraction of carboxyl groups in the oxidized regenerated cellulose described in Step 1 is 17.5%. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0038] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that in step one, the oxidized regenerated cellulose is immersed in 2-morpholineethanesulfonic acid with a molar concentration of 0.1mol / L, and then Ultrasonic treatment at 400W for 15min, then add 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide, then at room temperature and stirring speed React at 450 r / min for 15 minutes to obtain activated oxidized regenerated cellulose. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a preparation method of a RGD-M13 bacteriophage / oxidized regenerated cellulose composite hemostatic material, and relates to a preparation method of a hemostatic material. The invention aims to solve the problems that in the prior art, after the modification of conventional oxidized regenerated cellulose, the hemostatic time is slightly prolonged, while the mechanical strength and biological absorbability of oxidized regenerated cellulose are both reduced. The preparation method comprises the following steps: step one, activating oxidized regenerated cellulose; step two, soaking the activated oxidized regenerated cellulose in RGD-M13 bacteriophage suspension so as to obtain the RGD-M13 bacteriophage / oxidized regenerated cellulose composite hemostatic material. The prepared RGD-M13 bacteriophage / oxidized regenerated cellulose composite hemostatic material can be used to stop bleeding, and hemostatic time is shortened by 10.4 to 32.9%. The provided prepared method can obtain a RGD-M13 bacteriophage / oxidized regenerated cellulose composite hemostatic material.

Description

technical field [0001] The invention relates to a preparation method of a hemostatic material. Background technique [0002] Various accidents inevitably occur in daily life. During emergency treatment and operation, medical accidents or even death may be caused by massive bleeding. Therefore, local and effective rapid hemostasis of patients is very important, and effective control of bleeding and reducing bleeding time have become important measures to reduce the mortality of patients. Commonly used clinical hemostatic materials such as hemostatic gauze, hemostatic fiber, and hemostatic bandage have limitations in use, such as longer hemostasis time, easy adhesion to the wound and difficult dressing change, and powerlessness to wound infection and suppuration. Rapid hemostasis and functional hemostasis will be the development direction of hemostatic drugs in the future. [0003] Commonly used absorbable hemostatic materials include fibrin glue, gelatin sponge, oxidized ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/28A61L15/20A61L15/36A61L15/42
CPCA61L15/20A61L15/28A61L15/36A61L15/42A61L2400/04
Inventor 吴亚东黄玉东毛传斌王芳
Owner HARBIN INST OF TECH
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