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Sub-totipotent stem cell special medium and special culture method thereof

A technology of subtotipotent stem cells and culture methods, applied in the field of subtotipotent stem cell culture

Active Publication Date: 2019-01-25
钜威细胞(厦门)医学科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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And serum-free for convenience

Method used

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  • Sub-totipotent stem cell special medium and special culture method thereof
  • Sub-totipotent stem cell special medium and special culture method thereof
  • Sub-totipotent stem cell special medium and special culture method thereof

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Embodiment Construction

[0024] The experimental methods in the following examples are conventional methods unless otherwise specified. The present invention will be described in further detail below.

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Abstract

The invention provides a special culture medium and a special culture method for sub-totipotent stem cells. Enough stem cells can be obtained through one passage, and are stored. The culture medium contains no serum, and can be conveniently used. The special culture medium for the sub-totipotent stem cells includes 95% of DMEM / F12, 5% of a serum substitute, 5mg / ml of human albumin, 5.5[mu]g / ml of linoleic acid, 10ng / ml of a basic fibroblast growth factor, 2*10<-8>mol / ml of dexamethasone, 10ng / ml of a human platelet-derived growth factor, 10ng / ml of an epidermal growth factor, 50[mu]mol / L of beta-mercaptoethanol and 2mmol / L of L-glutamine. The culture medium contains no serum, has a good culture effect, and allows a required number of frozen sub-totipotent stem stems to be reached only through culturing one generation.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to a culture of subtotipotent stem cells. Background technique [0002] Stem cells are a kind of primitive cells with self-renewal ability and multi-lineage differentiation potential under suitable microenvironment. Stem cells can be divided into embryonic stem cells, neonatal stem cells, and adult stem cells according to the order in which they appear during individual development, and can be divided into totipotent stem cells, subtotipotent stem cells, pluripotent stem cells, and unipotent stem cells according to their differentiation potential. At present, stem cells have been used to treat myocardial infarction, lupus erythematosus, rheumatoid arthritis, nerve damage and muscle atrophy. [0003] Subtotipotent stem cells refer to a class of stem cell subsets with the potential to differentiate into three germ layers. Under specific circumstances, they can theoretically ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A01N1/02
Inventor 王军霞黄福来
Owner 钜威细胞(厦门)医学科技有限公司
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