A drug that reverses drug resistance in refractory epilepsy

A technology for intractable epilepsy and drug resistance, applied in drug combinations, reverse RNA viruses, microorganisms, etc., can solve epilepsy multidrug resistance, anti-epileptic drugs cannot work effectively, and drug concentration in epilepsy lesions is reduced, etc. problem, to achieve the effect of good clinical application prospects, reduction of pumping rate, and reversal of drug resistance

Active Publication Date: 2019-03-01
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have confirmed that most antiepileptic drugs such as phenytoin and sodium valproate are natural substrates of P-gp, and P-glycoprotein actively pumps antiepileptic drugs out of the cell through energy consumption, resulting in the release of drugs in epilepsy lesions. The reduction of the concentration of antiepileptic drugs can not effectively play a role, which may be one of the important mechanisms causing multidrug resistance in refractory epilepsy

Method used

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  • A drug that reverses drug resistance in refractory epilepsy
  • A drug that reverses drug resistance in refractory epilepsy
  • A drug that reverses drug resistance in refractory epilepsy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Construction of recombinant lentivirus

[0018] 1. Test materials

[0019] DMEM medium + 10% FBS

[0020] D-Hank's Solution

[0021] Trypsin-EDTA Solution (0.25% Trypsin + 0.1 mM EDTA, Hyclone)

[0022] 10cm Petri Dish (Corning)

[0023] 15cm Petri Dish (Corning)

[0024] RNAi-mate (Shanghai Jima)

[0025] Serum-free DMEM medium

[0026] 50ml centrifuge tube (Corning)

[0027] Filter (Sartorius Stedim)

[0028] Centrifuge (TGL)

[0029] DMEM medium + 10% FBS

[0030] D-Hank's Solution

[0031] Trypsin-EDTA Solution (0.25% Trypsin + 0.1 mM EDTA, Hyclone)

[0032] 96-well plate (Corning)

[0033] Polybrene (5ug / ml Sigma)

[0034] Lentivirus-related plasmids were purchased from Shanghai GenePharma Co., Ltd.

[0035] name

model

Manufacturer

PCR machine

MG96+

Hangzhou Longkey Scientific Instrument Co., Ltd.

Refrigerated centrifuge

TGL-20M

Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.

Constant...

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Abstract

The invention discloses a nucleotide sequence shown in SEQ ID NO.1, and further discloses a recombinant virus containing the nucleotide sequence and application thereof. The recombinant lentivirus can inhibit expression of drug resistance protein Pgp and a drug-resistance related gene HIF-1alpha and also can decrease the pumping rate of a drug, can reverse the drug resistance of intractable epilepsy, can be jointly used with other drugs for treating epilepsy to treat the intractable epilepsy and is good in clinical application prospect.

Description

technical field [0001] The present invention relates to a drug for reversing drug resistance of intractable epilepsy. Background technique [0002] Refractory epilepsy, also known as intractable epilepsy, usually refers to the absence of progressive disease or space-occupying lesions of the central nervous system, but clinical delay. Refractory epilepsy can be determined only when the patient can tolerate the maximum dose, and the blood concentration reaches the effective range, but the seizure cannot be controlled, and the daily life is affected. Intractable epilepsy accounts for about 20% to 30% of epilepsy patients. [0003] According to the current research, the reason why the intractable epilepsy cannot be effectively treated is related to its drug resistance. Therefore, it is very important to find drugs that can reverse its drug resistance in order to achieve effective treatment. [0004] In the drug resistance of epilepsy, the most studied is the P-glycoprotein (P-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N7/01A61K48/00A61P25/08
CPCA61K48/005C12N7/00C12N15/113C12N2740/15041
Inventor 陈蕾冯培民
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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