Drug for reversing drug resistance of intractable epilepsy

A technology for refractory epilepsy and drug resistance, applied in drug combinations, retroRNA viruses, microorganisms, etc., can solve multidrug resistance in epilepsy, decreased drug concentration in epilepsy lesions, and ineffective antiepileptic drugs, etc. problems, to achieve good prospects for clinical application, reduce the pumping rate, and reverse the effect of drug resistance

Active Publication Date: 2016-04-13
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have confirmed that most antiepileptic drugs such as phenytoin and sodium valproate are natural substrates of P-gp, and P-glycoprotein actively pumps antiepileptic drugs out of the cell through energy consumption, resulting in the release of drugs in epilepsy lesions. The reduction of the concentration of antiepileptic drugs can not effectively play a role, which may be one of the important mechanisms causing multidrug resistance in refractory epilepsy

Method used

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  • Drug for reversing drug resistance of intractable epilepsy
  • Drug for reversing drug resistance of intractable epilepsy
  • Drug for reversing drug resistance of intractable epilepsy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 Construction of recombinant lentivirus

[0018] 1. Test materials

[0019] DMEM medium+10%FBS

[0020] D-Hank's Solution

[0021] Trypsin-EDTA Solution (0.25% Trypsin+0.1mMEDTA, Hyclone)

[0022] 10cm petri dish (Corning)

[0023] 15cm petri dish (Corning)

[0024] RNAi-mate (Shanghai Gemma)

[0025] Serum-free DMEM medium

[0026] 50ml centrifuge tube (Corning)

[0027] Filter (Sartorius Stedim)

[0028] Centrifuge (TGL)

[0029] DMEM medium+10%FBS

[0030] D-Hank's Solution

[0031] Trypsin-EDTA Solution (0.25% Trypsin+0.1mMEDTA, Hyclone)

[0032] 96-well plate (Corning)

[0033] Polybrene (5ug / mlSigma)

[0034] Lentivirus-related plasmids were purchased from Shanghai GenePharma Co., Ltd.

[0035] name model factory PCR instrument MG96+ Hangzhou Langji Scientific Instrument Co., Ltd. Refrigerated centrifuge TGL-20M Shanghai Luxiangyi Centrifuge Instrument Co., Ltd. Constant temperature shaking incubator ...

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Abstract

The invention discloses a nucleotide sequence shown in SEQ ID NO.1, and further discloses a recombinant virus containing the nucleotide sequence and application thereof. The recombinant lentivirus can inhibit expression of drug resistance protein Pgp and a drug-resistance related gene HIF-1alpha and also can decrease the pumping rate of a drug, can reverse the drug resistance of intractable epilepsy, can be jointly used with other drugs for treating epilepsy to treat the intractable epilepsy and is good in clinical application prospect.

Description

technical field [0001] The present invention relates to a medicine for reversing the drug resistance of intractable epilepsy. Background technique [0002] Intractable epilepsy, also known as intractable epilepsy, usually refers to no progressive disease or space-occupying lesions of the central nervous system, but clinically protracted, after more than 2 years of regular antiepileptic treatment, trial of main antiepileptic drugs alone or in combination, reaching Refractory epilepsy can only be determined when the patient can tolerate the maximum dose, the blood drug concentration reaches the effective range, and the seizures cannot be controlled and daily life is affected. Refractory epilepsy accounts for about 20% to 30% of epilepsy patients. [0003] According to current research findings, the reason why intractable epilepsy cannot be effectively treated is related to its drug resistance, so it is very important to find drugs that can reverse drug resistance to achieve e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N7/01A61K48/00A61P25/08
CPCA61K48/005C12N7/00C12N15/113C12N2740/15041
Inventor 陈蕾冯培民
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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