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An immunoturbidimetric detection kit for fibrinogen

A fibrinogen and detection kit technology, applied in biological testing, measuring devices, material testing products, etc., can solve problems such as poor stability, low sensitivity, and limited popularization and application, so as to improve analytical sensitivity and enhance stability. sexual effect

Inactive Publication Date: 2016-05-04
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, common fibrinogen immunoturbidimetric assay reagents have poor stability and low sensitivity, which limits its clinical application.

Method used

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  • An immunoturbidimetric detection kit for fibrinogen
  • An immunoturbidimetric detection kit for fibrinogen
  • An immunoturbidimetric detection kit for fibrinogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A conventional detection kit for fibrinogen immunoturbidimetric method, which comprises reagent R1, reagent R2 and calibrator.

[0028] Wherein the reagent R1 consists of:

[0029] pH6.8 Tris buffer 100mmol / L

[0030] Sodium azide 0.1%

[0031] Reagent R2 consists of:

[0032] pH6.8 Tris buffer 100mmol / L

[0033] Goat anti-human Fb antibody 30ml / L

[0034] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0035] Add 5 μl of physiological saline, sample or calibrator, then add 225 μl of R1 reagent for pre-incubation for 5 minutes and read the absorbance A1, then add 75 μl of reagent R2 and react for 5 minutes, read the absorbance A2, and calculate ΔA.

[0036] The calibrator used in this example is the Fb calibrator produced by Jiufeng Runda Biotechnology Co., Ltd.

Embodiment 2

[0038] A fibrinogen immune turbidimetric detection kit comprises reagent R1, reagent R2 and calibrator.

[0039] Wherein the reagent R1 consists of:

[0040] pH6.8 Tris buffer 100mmol / L

[0041] Sodium azide 0.1%

[0042] Silica coated magnetic nanoparticles 0.1%

[0043] Reagent R2 consists of:

[0044] pH6.8 Tris buffer 100mmol / L

[0045] Goat anti-human Fb antibody 30ml / L

[0046] Bovine serum albumin 20g / L

[0047] Kathon-CG0.05%

[0048] Concrete determination method is with embodiment 1.

Embodiment 3

[0050] A fibrinogen immune turbidimetric detection kit comprises reagent R1, reagent R2 and calibrator.

[0051] Wherein the reagent R1 consists of:

[0052] pH6.8 Tris buffer 100mmol / L

[0053] Sodium azide 0.1%

[0054] Silica coated magnetic nanoparticles 1%

[0055] Reagent R2 consists of:

[0056] pH6.8 Tris buffer 100mmol / L

[0057] Goat anti-human Fb antibody 30ml / L

[0058] Bovine serum albumin 30g / L

[0059] Kathon-CG0.05%

[0060] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses an immunoturbidimetric detection kit for fibrinogen, and belongs to the technical field of clinical in-vitro detection reagents. The kit comprises a reagent R1, a reagent R2 and a calibrating product. By adding a certain amount of silica-covered magnetic nanometer particles into the reagent R1 and adding a certain amount of bovine serum albumin and a certain amount of Kathon-CG into the reagent R2, stability and analysis sensitivity of the kit are effectively improved, and the kit is good in linear range and high in accuracy, and is beneficial to further popularization and application in the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a fibrinogen immunoturbidimetric detection kit. Background technique [0002] Fibrinogen is a protein synthesized by the liver with blood clotting function and is the precursor of fibrin. The molecular weight is 340,000, and the half-life is 4 to 6 days. The reference value in plasma is 2-4 g / L. Fibrinogen consists of three pairs of different polypeptide chains α, β, and γ, and the polypeptide chains are connected by disulfide bonds. Under the action of thrombin, the α-chain and β-chain release peptide A and peptide B, respectively, to generate fibrin monomers. During this process, due to the release of acidic peptides, the negative charge decreases, and the monomers are easy to polymerize into fibrin polymers. But at this time, the monomers are connected by hydrogen bonds and hydrophobic bonds, and can still be dissolved in dilute acid and urea s...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/68G01N33/54326G01N33/54346G01N2333/75
Inventor 甘宜梧董雯李静
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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