Seedling inoculation method for identification of resistance to Botrytis cinerea

Abstract

The invention relates to a gray mold seedling-stage inoculation identification method for capsicum and belongs to the technical field of plant protection. The method comprises the steps of culturing botrytis cinerea, collecting botrytis cinerea spores, preparing a 10<6> / mL botrytis cinerea spore suspension, sowing capsicum seeds in sterilized soil, putting the capsicum seeds into a greenhouse with the temperature of 20-25 DEG C, dropwise adding the spore suspension to seedling center growth points for inoculation after each seedling grows to a four-leaf period, carrying out closed moisture preserving on the greenhouse, and carrying out gray mold investigation 21 days later. The method has the technical characteristics of seedling stage identification, simplicity and convenience in operation, high efficiency, rapidness and accurate and reliable identification results. The method is mainly applied to the fast screening of disease-resistant germplasm from capsicum resource varieties and the disease resistance identification on the varieties and serves the selective breeding of disease-resistant new varieties and the rational layout of disease-resistant varieties.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for inoculating and identifying capsicum botrytis at the seedling stage, especially a method for screening capsicum botrytis-resistant resource materials. Background technique [0002] In recent years, with the adjustment of the rural industrial structure, the cultivation of pepper greenhouses in my country has developed rapidly. Due to the low temperature, high humidity and long duration of condensation in the greenhouse in winter and spring, it is very suitable for the occurrence of Botrytis cinerea. Therefore, the disease has become one of the most important diseases in the production of peppers in greenhouses in my country. If the disease loss is mild, the yield will be reduced by 15% to 20%, and if it is severe, the yield will be reduced by more than 30%. But so far, there are few studies on Botrytis cinerea, and the research on the identification of resis...

AI Technical Summary

Problems solved by technology

Botrytis cinerea can cause damage at both the seedling stage and the adult plant stage, while gray mold at the seedling stage often causes the death of the whole plant, or even death in pieces, resulting in more serious consequences

Although the in vitro inoculation method is simple, because the pepper plants are young at the seedling stage, Botrytis cinerea is a facultative parasite, but its saprophytic ability is extremely strong, and the in vitro inoculation results often cannot correctly distinguish the level of resistance, nor can it reflect the pepper's resistance to infection. The resistance situation of Botrytis cinerea live infection