Method of assistant efficient breeding of corns cooperatively using two molecular markers of SSR and SNP

A molecular marker, corn technology, applied in the field of molecular biology and genetic breeding, can solve the problems of difficult to achieve high-throughput sites and high detection costs

Active Publication Date: 2016-05-25
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

PCR-based SSR markers have the advantages of high polymorphism, simple operation, and good repeatability, but it is difficult to achieve high throughput of sites
Sequence-based SNP markers have high distribution density, good genetic stability, and are easy to achieve high-throughput and automated analysis, but the detection cost is relatively high

Method used

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  • Method of assistant efficient breeding of corns cooperatively using two molecular markers of SSR and SNP
  • Method of assistant efficient breeding of corns cooperatively using two molecular markers of SSR and SNP
  • Method of assistant efficient breeding of corns cooperatively using two molecular markers of SSR and SNP

Examples

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Embodiment 1

[0033] Example 1 Method for using two molecular markers of SSR and SNP in combination to carry out corn assisted high-efficiency breeding

[0034] 1Materials and methods

[0035] 1.1 Test materials

[0036] The maize inbred line Jing SD121 has high combining ability and excellent comprehensive characters, but has the disadvantages of high ear position and poor lodging resistance. With low ear position and good lodging resistance, the inbred line Jing SD127, which is closely related to Jing SD121, was used as the donor parent, and Jing SD121 was used as the recurrent parent to improve the lodging resistance of Jing SD121 through backcrossing. In this example, 157 BC strains initially selected by field phenotype selection were selected. 1 F 1 The generation backcross group is the research material.

[0037] 1.2 DNA extraction

[0038] The seedlings of Jing SD127 and Jing SD121 were grown in indoor sand culture, and DNA was extracted at the 3 to 5 leaf stage. BC 1 F 1 Generation field s...

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Abstract

The invention relates to a method of assistant efficient breeding of corns cooperatively using two molecular markers of SSR and SNP. The corn selfing line Jing SD127 is adopted as a donor parent while Jing SD121 is adopted as a recurrent parent; a BC1F1-generation group is obtained through backcross transformation; 6 SSR markers and 104 SNP markers are adopted for background selection for the BC1F1-generation group; and single plants with high background recovery rate are screened to increase the improvement speed of the corn selfing line Jing SD121. According to comparison of the background selection results of the SSR and SNP markers, the two markers are correlated in a middle degree. Therefore, the background selection can be performed by combining the SSR and SNP markers in the backcross early generation so as to realize large-group strict selection and improve the selection efficiency.

Description

Technical field [0001] The invention relates to the fields of molecular biology and genetic breeding, and specifically relates to a method for using two molecular markers, SSR and SNP, to assist efficient maize breeding. Background technique [0002] Conventional maize breeding selects target individuals through phenotypic observation, which not only relies on the experience of the breeder, but is also often affected by the interaction of genotype and environment, making it difficult to select the ideal genotype. Molecular marker-assisted selection is an auxiliary means of applying molecular markers to selection in the process of crop variety improvement. It mainly includes foreground selection of target traits and background selection of genetic background. Using molecular markers to assist background selection in the process of backcrossing can significantly speed up the background recovery and shorten the breeding time. [0003] Molecular markers suitable for background selecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A01H1/04A01H1/02
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/156
Inventor 赵久然宋伟王凤格田红丽易红梅葛建镕李瑞媛王元东段民孝赵衍鑫张如养李春辉
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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