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Root wetting method using middle-concentration NAA (Naphthalene Acetic Acid) and glycerin gel

A technology of glycerol gel and concentration, applied in the field of plant biology, can solve problems such as high cost, loss of branches, instability, etc., and achieve the effects of easy operation and reduction of water evaporation

Inactive Publication Date: 2016-06-08
何晴雪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] A method for dipping roots with medium-concentration NAA and glycerin gel, characterized in that the method includes hormone mother liquor preparation, working solution preparation, hormone gel preparation, root dipping, and cuttings, and the specific steps are as follows:

[0014] ① Hormone mother solution preparation: Accurately weigh 0.1g of NAA and dissolve it in a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL NAA mother solution;

[0015] ②Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:10 into a medium-concentration hormone working solution with a concentration of 100mg / L, and add 10ml of glycerin to 90ml of hormone working solution at the same time;

[0016] ③ Hormone gel preparation: Accurately weigh 1g of sodium carboxymethylcellulose and 8g of sodium alginate, and dissolve them in 100mL of hormone and glycerol working solution with a concentration of 100mg / L in a water bath at...

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PUM

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Abstract

The invention discloses a root wetting method using middle-concentration NAA (Naphthalene Acetic Acid) and glycerin gel and belongs to the field of plant biotechnology. The preparation method comprises the following steps: firstly, preparing 1mg / mL NAA mother solution; diluting the mother solution into 100mg / L middle-concentration NAA working solution; preparing middle-concentration NAA and glycerin gel confining liquid containing sodium carboxymethylcellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8% from the working solution above; dipping the lower ends of healthy and thick branches in the gel confining liquid for 3-5 seconds and then taking out; and inserting the branches into a cultivation matrix and rooting.

Description

technical field [0001] The invention discloses a method for dipping roots with medium-concentration NAA and glycerin gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as NAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N37/10A01P21/00A01G9/10A01G7/06
CPCA01N37/10A01G7/06A01G9/0299A01N25/04
Inventor 何晴雪
Owner 何晴雪