A method for amplifying and activating lak cells without fetal bovine serum

A technology of fetal bovine serum and NK cells, applied in the field of immunology, can solve the problems of difficulty in obtaining high purity, low purity of LAK cells, low killing function of CIK cells, etc., to achieve the effect of resisting viruses and bacteria and improving the killing ability

Active Publication Date: 2020-08-25
ZHEJIANG UNIV
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Problems solved by technology

The number and killing function of LAK cells are directly related to the curative effect. The problem in the treatment is that it is very difficult to obtain high-purity and sufficient numbers of CIK cells and NK cells
The purity of LAK cells expanded by traditional serum-free medium is low (20%-30%); the killing function of expanded CIK cells is low, which cannot meet clinical needs

Method used

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  • A method for amplifying and activating lak cells without fetal bovine serum
  • A method for amplifying and activating lak cells without fetal bovine serum
  • A method for amplifying and activating lak cells without fetal bovine serum

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Embodiment Construction

[0040] The invention discloses a method for amplifying and activating lymphocytes without fetal bovine serum, using host cells transfected with protein A, protein B, protein C, protein D, protein E, and protein F, combined with various proteins to stimulate lymphocytes Perform expansion and activation to obtain LAK lymphocytes; the host cells after transfection play an important role in the expansion and activation of lymphocytes, and sufficient number and function of LAK cells can be obtained, which can recognize and destroy tumor microenvironment Tumor cells, M2-type monocytes and CD19+ B cells enhance the patient's immune response. The protein A contains the functional fragments of CD8α and interleukin 21; the protein B contains the functional fragments of CD14; the protein C contains the functional fragments of CD19; the protein D contains the functional fragments of CD86; the protein E contains the functional fragments of CD163; Contains the functional fragment of CD137L;...

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Abstract

The invention discloses a method for amplifying and activating LAK cells by non-fetus bovine serum. The method has the advantage that compared with the existing fetal bovine medium, the risk brought by adopting fetus bovine serum can be avoided; compared with the existing serum-free medium, the purity of amplified and activated immune cells is higher, the amplification efficiency is higher, and the ability to kill tumor is stronger. The purity of amplified and activated immune cells, the amplification efficiency, and the ability to kill tumor by the method are equivalent to those of the existing fetal calf serum medium. The immune cells amplified by the method on the aspects of controlling and relieving progresses of a part of diseases, such as cancer, communicable disease, diabetes, or organ transplantation and hepatitis B have a wide prospect.

Description

technical field [0001] The invention belongs to the field of immunology, and specifically refers to a method for directional amplification and activation of LAK cells by using host cells transfected with various proteins and combining multiple proteins to act together. Background technique [0002] LAK cells are a type of cell population generated by the activation of lymphokines, including CIK, NK, NKT, T and other cells. LAK cell therapy has a significant effect on a variety of tumors, such as lung cancer, squamous cell carcinoma of the head and neck, sarcoid carcinoma and acute leukemia. At present, LAK cell therapy is mainly used in combination with surgery, chemotherapy and radiotherapy to solve the problem of tumor recurrence. The number and killing function of LAK cells are directly related to the curative effect. The problem in the treatment is that it is very difficult to obtain high-purity and sufficient numbers of CIK cells and NK cells. The purity of LAK cells ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783A61K35/17A61P35/00A61P3/10
CPCA61K35/17C12N5/0638C12N2501/2301C12N2501/2302C12N2501/2321C12N2501/24C12N2501/505C12N2501/51C12N2501/515C12N2501/599
Inventor 徐以兵郑树梁廷波朱莉莉胡薇蕾
Owner ZHEJIANG UNIV
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