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A method for expanding and activating inkt lymphocytes

A technology of lymphocytes and cells, applied in the field of immunology, can solve the problems of limited multiples, difficulties, and low purity, and achieve the effects of resisting viruses and bacteria, improving killing ability, and improving immunity

Active Publication Date: 2020-04-24
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

The number and killing function of iNKT cells are directly related to the curative effect. The problem in the treatment is that it is very difficult to obtain a large number of high-purity iNKT cells
The traditionally used interleukin 2 combined with α-galactosylceramide has a limited growth factor (average 100 times); the purity is low (20%-30%); the amplified iNKT cells have low killing function and cannot meet clinical needs

Method used

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  • A method for expanding and activating inkt lymphocytes
  • A method for expanding and activating inkt lymphocytes
  • A method for expanding and activating inkt lymphocytes

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Embodiment Construction

[0029]The invention provides a method for amplifying and activating iNKT cells. The method uses host cells transfected with protein A, protein B, protein C, protein D, protein E, and protein F, combined with protein A1 and α-galactosylceramide Expand and activate lymphocytes together to obtain iNKT lymphocytes; the host cells after transfection play an important role in the expansion and activation of lymphocytes, and sufficient numbers and functions of iNKT cells can be obtained to recognize and destroy tumors Tumor cells, M2-type monocytes and CD19+ B cells in the microenvironment enhance the patient's immune response. The protein A contains a complete fragment of CD8α and a functional fragment of interleukin 21; protein B contains a functional fragment of CD14; protein C contains a functional fragment of CD19; protein D contains a functional fragment of CD86; protein E contains a functional fragment of CD163; Contains the functional fragment of CD137L; Among them, the CD8α ...

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Abstract

The invention discloses a method for amplifying and activating an iNKT (invariant natural killer T) lymphocyte by using a host cell of transfection functional protein and associating with multiple kinds of protein. Compared with an existing method, the amplification and activation method of the iNKT lymphocyte has the advantages that the purity of an iNKT cell amplified and activated by the amplification and activation method of the iNKT lymphocyte is higher; the amplification efficiency is higher; the capacity of killing a tumor and TAMs (Tumor-Associated Macrophages) in a tumor microenvironment is stronger. The amplification and activation method of the iNKT lymphocyte has a wide prospect in the progress of controlling and alleviating part of diseases, such as the rejection aspects of the tumor, diabetes and organ transplantation.

Description

technical field [0001] The invention belongs to the field of immunology, and specifically refers to a method for directional amplification and activation of iNKT cells by using host cells transfected with various proteins and combining multiple proteins to act together. Background technique [0002] iNKT cell therapy has a significant effect on a variety of tumors such as lung cancer, squamous cell carcinoma of the head and neck, sarcoid carcinoma and acute leukemia. At present, iNKT cell therapy is mainly used in combination with surgery, chemotherapy and radiotherapy in the United States to solve the problem of tumor recurrence. The number and killing function of iNKT cells are directly related to the curative effect. The problem in the treatment is that it is very difficult to obtain a large number of high-purity iNKT cells. The traditionally used interleukin 2 combined with α-galactosylceramide has a limited growth rate (average 100 times); the purity is low (20%-30%); ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783A61K35/17A61P35/00A61P3/10A61P43/00
CPCA61K35/17C12N5/0646C12N2500/36C12N2501/2302C12N2501/2321C12N2501/505C12N2501/51C12N2501/599
Inventor 徐以兵郑树梁廷波朱莉莉胡薇蕾
Owner ZHEJIANG UNIV
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