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Application of diosgenin in the preparation of drugs for promoting liver cell regeneration

A technology of diosgenin and liver cell promotion, which is applied in the application field of diosgenin in the preparation of drugs for promoting liver cell regeneration, and can solve the problems of no diosgenin, etc.

Active Publication Date: 2019-03-15
DALIAN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no relevant report on the application of diosgenin in promoting liver cell proliferation and in the preparation of drugs for promoting liver cell regeneration

Method used

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  • Application of diosgenin in the preparation of drugs for promoting liver cell regeneration
  • Application of diosgenin in the preparation of drugs for promoting liver cell regeneration
  • Application of diosgenin in the preparation of drugs for promoting liver cell regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Study on the Effect of Dioscin on the Regeneration of Rat Hepatic Primary Cells

[0022] 1. Experimental method

[0023] Rat hepatocytes were isolated by collagenase in situ perfusion. Ether was inhaled to anesthetize the rat, the whole body was disinfected with Promethicone, the abdomen of the rat was opened in a U shape, the portal vein was intubated and fixed, the inferior vena cava was quickly cut, and the liver was perfused with 0.02% calcium-free EDTA-PBS solution at 37°C until the liver completely turned khaki , followed by perfusion with 0.025% Ⅳ collagenase solution at 37°C until the liver becomes soft, separate the liver, tear the liver capsule, collect the liver cells into the DMEM washing solution at 4°C, filter through a 200-mesh gauze screen, and let it stand , low-speed centrifugation (600r / min, 5min) repeated 3 times, inoculated with cell culture medium and cultured in the culture bottle. Rat primary hepatocytes were cultured in DMEM medium (...

Embodiment 2

[0027] Example 2 Study on the effect of diosgenin on the regeneration of rat AML12 liver cells

[0028] 1. Experimental method

[0029] AML12 cells were cultured in DMEM / F-12 complete medium containing 1% ITS, 40ng / mL dexamethasone and 10% fetal bovine, and the medium was changed every 24 hours. Before giving diosgenin treatment, the cells were plated in 96-well plates and grown for 24 hours. After giving different concentrations of diosgenin for different times (6, 12 and 24 hours), the cell viability, Brdu cell proliferation, and PCNA proliferation antigen staining were measured. To evaluate the effect of drugs on the proliferation of AML12 cells in vitro.

[0030] 2. Results

[0031] The effect of diosgenin on the proliferation of rat liver AML12 cells, see image 3 and Figure 4 . Depend on image 3 The results of MTT assay showed that when 150ng / ml, 300ng / ml and 600ng / ml diosgenin acted on AML12 cells, the cell viability was significantly enhanced (p Figure 4 The resu...

Embodiment 3

[0033] Example 3 Study on Effects of Dioscin on Liver Regeneration in C57BL / 6 Mouse Partial Hepatectomy Model

[0034] 1. Animal experiments

[0035] Male C57BL / 6 mice were taken and fed with diosgenin at doses of 40 mg / kg and 80 mg / kg continuously for 7 days. On the 4th, 5th, 6th, and 7th day of administration, 70% hepatectomy was performed to establish a mouse model of partial hepatectomy, and all mice were sacrificed on the seventh day for experimental determination.

[0036] 2. Liver regeneration index

[0037] After fasting for 12 hours after the last administration, the liver weight and body weight of the mice were weighed, and the liver regeneration index was calculated based on the ratio of the two.

[0038] 3. Liver tissue biochemical index detection

[0039] After fasting for 12 hours after the last administration, blood was taken to determine the contents of ALT and AST in serum

[0040] 4. Pathological examination of liver tissue

[0041] (1) H&E staining of l...

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Abstract

The invention discloses application of dioscin which has the effect of promoting hepatic cell regeneration and can be used for preparing medicine for promoting hepatic cell regeneration.The dioscin can significantly enhance the hepatic cell activity, promote hepatic cell proliferation and increase the hepatic regeneration index after partial hepatectomy and has the significant down-regulation effect on ALT and AST increasing caused by the partial hepatectomy.After intervening is performed with the dioscin, hepatic cell microbubble degeneration and hepatic structure disorder phenomena which are caused by the partial hepatectomy are obviously reduced; in addition, it is found that the dioscin can significantly promote hepatic cell regeneration through BrdU and PCNA detection.The dioscin can be prepared into the medicine by serving as a single effective ingredient and also can be prepared into a compound preparation by being cooperatively used with other medicine according to the clinical medication requirements; the dioscin has the advantages of being safe (free of toxic and side effects), effective, economical, practical and the like.

Description

technical field [0001] The invention relates to a new application of active ingredients of traditional Chinese medicine, in particular to the application of high-purity diosgenin in the preparation of drugs for promoting liver cell regeneration. Background technique [0002] As the largest substantive organ in the human body, the liver plays a central role in maintaining metabolism in the body, and is an important organ with strong regenerative capacity. In normal liver tissue, most hepatocytes do not undergo mitosis and are in a relatively static state. Many chemicals, drugs, and viruses can cause liver damage, mainly affecting the body's immune response, inducing inflammation and necrosis of liver cells, steatosis, cholestasis, etc., and ultimately leading to a variety of acute and chronic liver injuries. However, partial hepatectomy is still the main treatment for various hepatobiliary tumors and acute and chronic end-stage liver diseases. After the liver is partially r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P1/16
CPCA61K31/7048
Inventor 彭金咏许丽娜尹连红齐艳许有威韩旭赵艳艳陶旭峰
Owner DALIAN MEDICAL UNIVERSITY
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