Preparation of biochar-loaded psb passivator and method for remediating pb-contaminated soil

A biochar and passivating agent technology, applied in the field of soil remediation, can solve the problems of easy loss of active PSB, phagocytosis, and poor repair effect, and achieve long-term stable passivation effect, stable properties, and convenient operation

Active Publication Date: 2019-03-19
INST OF HYDROGEOLOGY & ENVIRONMENTAL GEOLOGY CHINESE ACAD OF GEOLOGICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a biochar-loaded PSB passivation solution to solve the technical problem that the added active PSB is very easy to lose or be swallowed by indigenous microorganisms when the heavy metal pollution is repaired by using microbial technology in the prior art, thereby resulting in a poor repair effect. Preparation of agent and method for remediating Pb polluted soil

Method used

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  • Preparation of biochar-loaded psb passivator and method for remediating pb-contaminated soil
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  • Preparation of biochar-loaded psb passivator and method for remediating pb-contaminated soil

Examples

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Effect test

Embodiment 1

[0048] Preparation of biochar:

[0049] In the present invention, cow dung is selected as the raw material for preparing biochar. After the raw material is cleaned and air-dried, it is placed in a closed metal container to form an anoxic environment. It is heated at a constant temperature of 350°C in a muffle furnace for 2 hours, and ground for 60 minutes after natural cooling. Mesh sieve, as the target biochar dry storage for future use.

[0050] Screening and acclimatization of Pseudomonas chloropinus:

[0051] a. Screening, collecting 5g of the upper layer 5-10cm of soil in the heavy metal polluted soil caused by the sewage irrigation of the battery factory, placing it in a 250ml Erlenmeyer flask with 90ml of distilled water, and cultivating it in a constant temperature oscillator at 30°C for 24 hours. In the selective medium containing phosphate rock powder, cultivate for another 5 days to form the phosphate-dissolving bacteria group;

[0052] B, acclimation, get the lys...

Embodiment 2

[0060] Preparation of biochar:

[0061] In the present invention, cow dung is selected as the raw material for preparing biochar. After the raw material is washed and air-dried, it is placed in a closed metal container to form an oxygen-deficient environment. It is heated at a constant temperature of 330°C in a muffle furnace for 2.5 hours, and after natural cooling, it is ground for 60 Mesh sieve, as the target biochar dry storage for future use.

[0062] Screening and acclimatization of Pseudomonas chloropinus:

[0063] a. Screening, collecting 6g of the upper layer 5-10cm of soil in the heavy metal-contaminated soil caused by the sewage irrigation of the battery factory, placing it in a 250ml triangular flask with 90ml of distilled water, shaking and culturing it in a constant temperature oscillator at 28°C for 26 hours, and inoculating in In the selective medium containing phosphate rock powder, cultivate for another 5 days to form the phosphate-dissolving bacteria group;...

Embodiment 3

[0072] Preparation of biochar:

[0073] In the present invention, cow dung is selected as the raw material for preparing biochar. After the raw material is cleaned and air-dried, it is placed in a closed metal container to form an anoxic environment. It is heated at a constant temperature of 340°C in a muffle furnace for 2.1 hours, and ground for 60 hours after natural cooling. Mesh sieve, as the target biochar dry storage for future use.

[0074] Screening and acclimatization of Pseudomonas chloropinus:

[0075] a. Screening, collecting 7g of the upper layer 5-10cm of soil in the heavy metal-contaminated soil caused by the sewage irrigation of the battery factory, placing it in a 250ml Erlenmeyer flask with 90ml of distilled water, and cultivating it in a constant temperature oscillator at 29°C for 23 hours. In the selective medium containing phosphate rock powder, culture for another 6 days, which is the phosphate-dissolving bacteria group;

[0076] B, domestication, get t...

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Abstract

The invention discloses a preparation method of charcoal loaded PSB passivator, belongs to the technical field of soil restoration and further provides a method for restoring Pb polluted soil through the charcoal loaded PSB passivator. Green needle pseudomonades are adopted and immobilized; with charcoal as a carrier, the green needle pseudomonades cultured for the logarithmic phase are inoculated into a fermentation tank, charcoal is added for fermentation culture, then, immobilization is conducted, and the charcoal loaded PSB passivator is obtained. The preparation method is simple, easy to operation and suitable for large-scale production, the restoration capability of the charcoal loaded PSB passivator to soil is high, and the removing rate of lead is high.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, and relates to the remediation of soil by a biological passivator, in particular to the preparation of a biochar-loaded PSB passivator and a method for remediating Pb-contaminated soil. The preparation method of the biological charcoal-loaded PSB passivator of the invention is simple, easy to operate, and easy to produce on a large scale, and the method for repairing Pb-contaminated soil is simple and fast, and has strong repair ability. Background technique [0002] According to statistics, my country has 26.67 million hm 2 The cultivated land has been polluted by heavy metals to varying degrees, which has led to the degradation of soil fertility, the reduction of crop yield and quality, and threatened people's food safety. Typical characteristics of heavy metal-contaminated soils in my country. [0003] Among many soil heavy metal remediation technologies, the use of phosphorus-contai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10B09C1/10
CPCB09C1/10C12N11/10C12N11/14
Inventor 张学庆李亚松李卉费宇红
Owner INST OF HYDROGEOLOGY & ENVIRONMENTAL GEOLOGY CHINESE ACAD OF GEOLOGICAL SCI
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