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Preparation method and application of Newcastle disease chicken embryo inoculation vaccine

A technology for inoculating chicken embryos and Newcastle disease virus, which is applied to biochemical equipment and methods, antisense single-stranded RNA viruses, microorganisms, etc., can solve the problems of affecting the immune effect of vaccines, toxic and side effects of chicks, and small toxic and side effects, and achieves Save vaccine immunization costs, reduce labor costs, and have no toxic side effects

Inactive Publication Date: 2016-07-13
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The immune effect of inactivated vaccines is better, but it needs intramuscular injection, which is labor-intensive and time-consuming, and affects the quality of chickens; medium-virulence live vaccines have strong toxic and side effects on chicks; low-virulence live vaccines are more convenient to use and have less side effects. However, the requirements for cold storage are high, and the immune effect of the vaccine is often affected due to improper storage or use; and the existing vaccines cannot be used for chicken embryo immunization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method for chicken embryo vaccination, comprising the following steps:

[0020] Step 1: Inoculation. Dilute the Newcastle disease virus TS09-C strain production virus seed to 10 －3 , Inoculate well-developed 9-day-old SPF chicken embryos, inoculate 0.1ml into the allantoic cavity of each embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C without turning the eggs;

[0021] Step 2: Incubation and observation. After inoculation of the chicken embryos, eggs should be illuminated once a day, and the chicken embryos that died before 48 hours should be discarded. After 48 hours, eggs should be illuminated once every 4 to 8 hours. Whether it is dead or not, take them all out, put the air chamber upright, and cool at 2-8°C.

[0022] Step 3: Harvest. Take out the chicken embryos that have been cooled for 4 to 24 hours, disinfect the air chamber with tincture of iodine, and then use aseptic surgery to peel off the egg shell of the air ...

Embodiment 2

[0026] A preparation method for chicken embryo vaccination, comprising the following steps:

[0027] Step 1: Inoculation. Dilute the Newcastle disease virus TS09-C strain production virus seed to 10 －4 , Inoculate well-developed 10-day-old SPF chicken embryos, inoculate 0.1ml into the allantoic cavity of each embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C without turning the eggs;

[0028] Step 2: Incubation and observation. After inoculation of the chicken embryos, eggs should be illuminated once a day, and the chicken embryos that died before 48 hours should be discarded. After 48 hours, eggs should be illuminated once every 4 to 8 hours. Whether it is dead or not, take them all out, put the air chamber upright, and cool at 2-8°C.

[0029] Step 3: Harvest. Take out the chicken embryos that have been cooled for 4 to 24 hours, disinfect the air chamber with tincture of iodine, and then use aseptic surgery to peel off the egg shell of the air...

Embodiment 3

[0033] A preparation method for chicken embryo vaccination, comprising the following steps:

[0034] Step 1: Inoculation. Dilute the Newcastle disease virus TS09-C strain production virus seed to 10 －3 , Inoculate well-developed 10-day-old SPF chicken embryos, inoculate 0.1ml into the allantoic cavity of each embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C without turning the eggs;

[0035] Step 2: Incubation and observation. After inoculation of the chicken embryos, eggs should be illuminated once a day, and the chicken embryos that died before 48 hours should be discarded. After 48 hours, eggs should be illuminated once every 4 to 8 hours. Whether it is dead or not, take them all out, put the air chamber upright, and cool at 2-8°C.

[0036] Step 3: Harvest. Take out the chicken embryos that have been cooled for 4 to 24 hours, disinfect the air chamber with tincture of iodine, and then use aseptic surgery to peel off the egg shell of the air...

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Abstract

The invention discloses a preparation method and application of a Newcastle disease chicken embryo inoculation vaccine. The preparation method comprises the following steps: properly diluting a virus seed for producing a Newcastle disease virus TS09-C strain, and inoculating SPF chicken embryos which develop well and are 9 to 10 days old; inoculating 0.1ml to an allantoic cavity of each embryo; after inoculating, sealing a needle hole and continually hatching; illuminating eggs for one time every 4 to 8 hours; taking out dead chicken embryos at any time; taking out the chicken embryos, disinfecting an air chamber part with iodine tincture and sucking chicken embryo fluid; putting the collected chicken embryo fluid into a sterilization bottle and adding suitable antibiotics; after remaining a sample, freezing and preserving; filtering the plurality of groups of qualified chicken embryo fluid and mixing in the same container; adding sucrose degreased milk at a certain ratio as a stabilizing agent according to a regulated part of each chicken; meanwhile, adding the suitable antibiotics, quantitatively sub-packaging, and rapidly freezing and drying in vacuum. A chicken embryo immunization method disclosed by the invention has the advantages of safety, no toxic side effect, reduction of stress, good immune effect, low cost and the like.

Description

technical field [0001] The invention belongs to the technical field of chicken disease prevention and control, and in particular relates to a preparation method and application of Newcastle disease chicken embryo inoculation vaccine. Background technique [0002] The prevention and control of Newcastle disease is mainly based on immunization prevention. Inactivated vaccines, medium-virulence live vaccines (I line) and low-virulence live vaccines (II line, IV line, etc.) have been widely promoted and used at home and abroad. The immune effect of inactivated vaccine is better, but it needs intramuscular injection, which is labor-intensive and time-consuming, and affects the quality of chicken; the medium-virulence live vaccine has strong toxic and side effects on chicks; the low-virulence live vaccine is more convenient to use and has less side effects. However, the requirements for cold storage are high, and the immune effect of the vaccine is often affected due to improper ...

Claims

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Application Information

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IPC IPC(8): A61K39/17A61P31/14
CPCA61K39/12C12N2760/18134
Inventor 王红琳温国元邵华斌罗青平汪宏才罗玲张蓉蓉张腾飞艾地云卢琴
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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