A fed-batch two-layer cell co-culture organ chip

Abstract

The invention discloses a fed-batch type two-layer cell co-culture chip which comprises a chip slot, a porous membrane, a sleeve layer and a top cover, wherein the chip slot comprises a lower cell culture area and three uniformly distributed flow choking barriers; an upper culture liquid inlet, an upper reagent inlet, a lower culture liquid inlet, a lower reagent inlet, an upper outlet and a lower outlet are formed in the outer part of the chip slot; an upper cell culture area and three uniformly distributed U-shaped flow choking barriers are arranged on the front side of the sleeve layer; a sleeve layer gap and three fixed barriers are arranged on the back side of the sleeve layer. The fed-batch type two-layer cell co-culture chip is capable of co-culturing two layers of cells, particularly due to design of the porous membrane, cell non-contact type information communication of upper and lower layers of culture systems can be achieved, the growth situation of cells and drug toxicity or pharmacology activity can be detected at real time on line, and the fed-batch type two-layer cell co-culture chip has the characteristics of simplicity, portability, economy, efficiency and accuracy, and can be popularized to mutual action relationship of multiple organs and tissues, medicine screening and toxicity evaluation.

Description

technical field [0001] The invention relates to a cultured organ chip, in particular to a fed-batch type two-layer cell co-cultured organ chip. Background technique [0002] At present, in the field of cell biology, life science and related medical research under space microgravity and rotation conditions, most of the cytology experiments use cell culture flasks or cell culture containers of specific hard materials. This structure makes all cell samples only It can adopt a single culture and experiment mode, but cannot carry out personalized experiment management, and it is difficult to realize the research on the interaction of cells in multiple organ types. Co-culture technology is a culture system that is more similar to the interaction between organs in vivo, so that cells can communicate with each other, promote growth and proliferation, and induce cell differentiation, maintain cell function and vitality, regulate cell proliferation, and improve metabolites. Influence...

AI Technical Summary

Problems solved by technology

The indirect co-culture system enables different types of cells to share the same culture system without direct contact. At present, Transwell multi-well plates are mostly used in conventional experiments to study cell migration and interaction, but they cannot be applied to space microgravity and rotation conditions.

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