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Wind-cold-dampness arthralgia type knee osteoarthritis model building method and device and use method

A technology for knee osteoarthritis and wind-cold-damp arthralgia, which is applied in the field of animal model establishment, can solve problems such as failure to reflect the overall concept and dialectical treatment, difficulty in expressing drug effects efficiently and intuitively, unfavorable drug tests, etc., to improve efficiency Accuracy, reproducibility, and overcoming traumatic effects

Inactive Publication Date: 2016-07-20
SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE PUTUO DISTRICT CENT HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The following problems still exist in the existing technology: 1) the trauma caused by the existing technology is large, which is unfavorable for drug testing; Knee osteoarthritis caused by relatively large trauma belongs to the middle and late stages of knee osteoarthritis, and it is difficult to express the effect of drugs efficiently and intuitively
Therefore the prior art fails to reflect the overall concept and dialectical treatment based on the compatibility of prescriptions and medicines for the clinical treatment of osteoarthritis in traditional Chinese medicine

Method used

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  • Wind-cold-dampness arthralgia type knee osteoarthritis model building method and device and use method

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Embodiment Construction

[0031] In the following, the present invention will be described in detail and specifically through specific examples, so as to better understand the present invention, but the following examples do not limit the scope of the present invention.

[0032] 1. Embodiment

[0033] This embodiment provides a method for establishing a model of rat knee osteoarthritis of wind-cold-damp arthralgia type, comprising: Step 1, cutting off the anterior cruciate ligament of the rat knee joint to cause joint instability; Step 2, after 2 weeks, treating Step 1 The rats were placed in ice water for 1 hour of exercise every day for 4 consecutive weeks to obtain the knee osteoarthritis model of wind-cold-dampness type. The index detection methods in the second step include: observing the general shape of the articular cartilage with the naked eye, toluidine blue staining, type II collagen immunohistochemical staining, and RT-PCR detection to detect the expression of aggrecan and type II collagen ...

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Abstract

The invention discloses a wind-cold-dampness arthralgia type knee osteoarthritis model building method.The method comprises the following steps that 1, knee joint anterior cruciate ligaments of a rat are cut off, and joint instability is caused; 2, two weeks later, the rat treated in the first step is put into ice water for activity for one hour every day, the whole process is continuously conducted for four weeks, and a wind-cold-dampness arthralgia type knee osteoarthritis model is obtained.The invention further discloses a device used for establishing the model and a use method.According to the method and the device, operation is easy, the effect is definite, repeatability is high, the theory of modern medicine to osteoarthritis pathogenesis is combined with the traditional medicine heumatism etiological theory, and on the basis of mechanical instability of the knee joints of the rat, cold stimulation is added to serve as an additional factor for induction, and therefore the wind-cold-dampness arthralgia type knee osteoarthritis model based on the mechanical instability is built.

Description

technical field [0001] The invention belongs to the technical field of establishing animal models, and relates to a method for establishing a model of rat wind-cold-damp arthralgia knee osteoarthritis, in particular to a method for establishing a model of wind-cold-damp arthralgia knee osteoarthritis with a mechanical instability basis, Device and method of use thereof. Background technique [0002] Knee osteoarthritis (KOA) is a chronic disease based on progressive degeneration of articular cartilage. It is generally believed that the primary lesion is in the cartilage and gradually affects the synovium, subchondral bone, joint capsule and other tissues. Its main features are progressive cartilage degeneration, subchondral sclerosis, and osteophyte formation at the joint margins and secondary synovitis. With the aging of the population, the incidence and number of patients with KOA are increasing year by year. Since the mechanism causing cartilage degeneration is not yet ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D3/00A61D7/00
CPCA61D1/00A61D3/00A61D7/00
Inventor 周军杰纪斌吕顺
Owner SHANGHAI UNIV OF TRADITIONAL CHINESE MEDICINE PUTUO DISTRICT CENT HOSPITAL
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