Cell culture medium and cell culture method

A technology for cell culture and culture medium, applied in the field of cell culture medium and cell culture, can solve the problems of cumbersome process, inability to effectively solve the problems of large-scale cell culture and high cost, and achieve the effect of reducing cost and realizing large-scale culture.

Inactive Publication Date: 2016-08-10
CHENGDU YUANRUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, serum-free medium requires special training of cells and needs to be specially customized for cells. The

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] First, add RPMI 1640 medium to the culture flask, and add human keratinocyte growth factor, fibroblast growth factor, colony-stimulating factor and fibronectin to the basal medium.

[0045] Control the concentrations of human keratinocyte growth factor, fibroblast growth factor, colony-stimulating factor and fibronectin in the basal medium to 100IU / mL, 200pg / mL, 70ng / mL, 1pg / mL, respectively.

[0046] Next, the HaCaT cells were inoculated with a cell concentration of 5×10 4 cells / mL were inoculated into culture flasks.

[0047] Then, place the inoculated flask at 37°C, 5% CO 2 cultured in an incubator.

[0048] With the above-mentioned basal medium (0% FBS) that added the growth factor composition as the experimental group, with the basal medium (without adding the growth factor composition) containing 10% FBS as the control group, it was observed that the HaCaT cells were different in the two groups. growth in culture medium.

[0049] The results showed that after 72...

Embodiment 2

[0051] First, add M199 medium to the culture flask, and add human keratinocyte growth factor, fibroblast growth factor, colony-stimulating factor fibronectin and epidermal growth factor to the basal medium.

[0052] Control the concentration of human keratinocyte growth factor, fibroblast growth factor, colony-stimulating factor, fibronectin and epidermal growth factor in the basal medium to 1IU / mL, 500pg / mL, 50ng / mL, 60pg / mL, 50IU / mL, respectively .

[0053] Next, the HaCaT cells were inoculated with a cell concentration of 5×10 4 cells / mL were inoculated into culture flasks.

[0054] Then, place the inoculated flask at 37°C, 5% CO 2 cultured in an incubator.

[0055] With the above-mentioned basal medium (0% FBS) that added the growth factor composition as the experimental group, with the basal medium (without adding the growth factor composition) containing 10% FBS as the control group, it was observed that the HaCaT cells were different in the two groups. growth in cul...

Embodiment 3

[0058] First, add DMEM medium to the culture flask, and add human keratinocyte growth factor, fibroblast growth factor, colony-stimulating factor fibronectin and nerve growth factor to the basal medium.

[0059] Control the concentrations of human keratinocyte growth factor, fibroblast growth factor, colony-stimulating factor fibronectin and nerve growth factor in the basal medium to 30IU / mL, 350pg / mL, 1ng / mL, 100pg / mL, 500ng / mL, respectively.

[0060] Next, the HaCaT cells were inoculated with a cell concentration of 5×10 4 cells / mL were inoculated into culture flasks.

[0061] Then, place the inoculated flask at 37°C, 5% CO 2 cultured in an incubator.

[0062] With the above-mentioned basal medium (0% FBS) that added the growth factor composition as the experimental group, with the basal medium (without adding the growth factor composition) containing 10% FBS as the control group, it was observed that the HaCaT cells were different in the two groups. growth in culture med...

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PUM

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Abstract

The invention discloses a cell culture medium, comprising a basic culture medium and a growth factor composition added to the basic culture medium, wherein the basic culture medium includes 0-1% of serum. The growth factor composition includes human keratinocyte growth factor, fibroblast growth factor, colony stimulating factor and fibronectin. The cell culture medium is low in cost, can promote normal growth of cells, and is suitable for large-scale culture of cells. In addition, the invention also discloses a method for culturing cells by using the cell culture medium.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a cell culture medium and a cell culture method. Background technique [0002] At present, the adherent growth of animal cells is the basic requirement of cell culture, and the intervention of serum has always been necessary in cell culture to ensure the normal growth of cells. But serum also has a downside for cell growth. The high cost, restrictions on the purchase of imported serum, and the negative impact of serum on cells have led to continuous research and exploration of serum substitutes. [0003] After the previous serum-free medium was launched on the market, it caused a lot of repercussions. However, serum-free medium requires special training of cells and needs to be specially customized for cells. The process is cumbersome and the cost is high, which cannot effectively meet the needs of large-scale cell culture. Both serum culture and serum-free culture have b...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0037C12N5/0625C12N2500/90C12N2501/11C12N2501/113C12N2501/115C12N2501/117C12N2501/13C12N2501/22C12N2501/998C12N2533/52
Inventor 贾芳苗杨诗明邓飞
Owner CHENGDU YUANRUI BIOTECH CO LTD
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