A method for evaluating the developmental potential of oocytes by using the relative expression level of grim-19 in granulosa cells
A GRIM-19, relative expression technology, used in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve accurate developmental potential, easy operation, and reduce adverse environmental stimuli The effect of
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[0044] A method for evaluating the developmental potential of oocytes by using the relative expression of GRIM-19 in granulosa cells, wherein the relative expression of GRIM-19 in the granulosa cells refers to GRIM-19mRNA and internal reference β-actin (β- action) ratio indicates:
[0045] When the GRIM-19 / β-action value in the granulosa cells of oocytes used for fertilization is 0.16-0.35, the obtained oocytes are judged to be oocytes with high developmental potential, and the granulosa cells of oocytes used for fertilization Among them, when the GRIM-19 / β-action value is 0-0.12, the obtained oocytes are judged to be oocytes with low developmental potential.
[0046] In this example, the relative expression of GRIM-19 in granulosa cells was measured by the following steps:
[0047] 1. Collection of human granulosa cells:
[0048] Conventional ovulation induction plan, mainly long or short ovulation induction treatment, is used to monitor the size of follicles by B-ultrasoun...
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