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Marine polypeptide-selenium chelate preparation method and application

A polypeptide complex and chelate technology, applied in the biological field, can solve the problems of small lethal dose, toxic and side effects, and a small range of poisoning and required doses, and achieves the effects of easy absorption and high extraction rate

Inactive Publication Date: 2016-08-24
XIAMEN 139 BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of absorption and utilization of inorganic selenium enhancers is not ideal, the bioavailability is low, and it has strong toxic and side effects. , so its use is strictly limited

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0021] A preparation method of marine polypeptide-selenium chelate, comprising the steps of:

[0022] (1) Preparation of marine peptides: First, ultrafinely pulverize marine animals and plants such as seaweed, fish, shrimp and crab, abalone, sea oyster, clam and their leftovers, and then mix the crushed marine animals and plants and their leftovers with water Mix according to mass volume ratio 1:10-50 (g / mL), use 0.5-2wt% NaOH solution or KOH solution to adjust the pH to 8.0-11.0, after ultrasonic extraction for 10-60min, extract in 50-100℃ water bath for 60-120min , filtered, the filtrate was adjusted to pH 2.5-4.0 with hydrochloric acid for protein precipitation, centrifuged at 3000-6000r / min for 5-10min to obtain the precipitated protein, washed with water to neutrality and then centrifuged again, and the obtained precipitate was freeze-dried to obtain marine animals and plants and their leftovers The protein in the solution; then dissolve the obtained protein with water to...

Embodiment 1

[0028] (1) Ultrafinely pulverize Enteromorpha enteromorpha, mix it with water at a mass-volume ratio of 1:30 (g / mL), adjust the pH to 9.0 with 2wt% NaOH solution, ultrasonically extract for 10 minutes, then extract in a water bath at 60°C for 100 minutes, Filtrate, adjust the pH of the filtrate to 3.0 with hydrochloric acid for protein precipitation, centrifuge at 4000r / min for 10 minutes to obtain the precipitated protein, wash with water to neutrality and then centrifuge again, and freeze-dry the obtained precipitate to obtain Enteromorpha protein, and the extraction rate reaches 74.16%;

[0029] (2) Dissolve the obtained Enteromorpha protein in water to make the protein concentration reach 3.0wt%, and adjust the pH of the solution to 9.0 with NaOH solution, then keep the temperature at 50°C, add 3.0wt% neutral protease, hydrolyze for 90min, and then Inactivate the enzyme to make Enteromorpha polypeptide complex solution;

[0030] (3) Add 1mol / L sodium selenite solution to t...

Embodiment 2

[0033] (1) After crushing the crucian carp, mix it with water at a mass-volume ratio of 1:20 (g / mL) to refine the pulp so that the protein concentration reaches 1.0wt%, and adjust the pH of the solution to 7.0, and then keep the temperature at 40°C , add 1.0wt% compound protease, hydrolyze for 60min, and then inactivate the enzyme to make crucian carp polypeptide complex solution;

[0034] (2) Add 0.5mol / L sodium selenite solution to the crucian carp polypeptide complex solution obtained in step (1) at a volume ratio of 2:6, stir evenly, adjust the pH to 7.0, and continue to react at 60°C for 3.0h before cooling , centrifuged to remove impurities, then added 4 times the volume of the supernatant, 80wt% ethanol, let stand for 16h, centrifuged to remove the supernatant, and the resulting precipitate was dried at low temperature to obtain crucian carp polypeptide-selenium chelate;

[0035] (3) The prepared crucian carp polypeptide-selenium chelate is further made into various sna...

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Abstract

The invention discloses a preparation method and application of a marine polypeptide-selenium chelate. Firstly, the protein in marine animals and plants and their leftovers is extracted by alkali-dissolving acid precipitation method, or the marine animals and plants and their leftovers are crushed, After refining, add protease to the obtained protein or slurry for enzymolysis, inactivate enzymes, and prepare a marine polypeptide complex solution; then use selenium in inorganic sodium selenite to chelate marine polypeptides to obtain marine polypeptide-selenium chelates, The obtained marine polypeptide-selenium chelate can be further prepared into snack food, nutritional health product or medicine. The preparation process of the invention is simple; the prepared marine polypeptide-selenium chelate and its products have a unique chelation system and transport mechanism, are safe, non-toxic, easy to be absorbed, and can supplement amino acids and selenium at the same time, and will surely become a selenium supplement It provides a new idea for the application of marine animals and plants and their leftovers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and application of a marine polypeptide-selenium chelate. Background technique [0002] Selenium (Se) is an important mineral element essential to the human body and has important physiological functions. A large number of existing studies have shown that many diseases are closely related to the nutritional status of selenium. The human body mainly obtains selenium through diet, and the method of artificial selenium supplementation is oral selenium-containing preparations. The existing selenium-containing preparations are generally made of oral preparations with inorganic selenium compounds (sodium selenite and sodium selenate). However, the effect of absorption and utilization of inorganic selenium enhancers is not ideal, the bioavailability is low, and it has strong toxic and side effects. , so its use is strictly limited. Studies have shown tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/165A23L33/18
CPCA23V2002/00A23V2200/30A23V2250/1626A23V2250/55
Inventor 刘斌张凤萍刘青
Owner XIAMEN 139 BIOTECH CO LTD
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