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Method for constructing high-efficiency bio-artificial liver system by using microcapsule and expanded bed integrated technology

A technology of expanded bed and microcapsules, applied in the direction of supporting/immobilizing microorganisms, biochemical equipment and methods, dialysis systems, etc., can solve the problems of low efficiency of material exchange and material metabolism, and achieve the reduction of solid phase back-mixing and dead ends, The effect of reducing shear damage and reducing breakage

Active Publication Date: 2016-08-24
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to overcome the shortcoming of low material exchange and material metabol

Method used

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  • Method for constructing high-efficiency bio-artificial liver system by using microcapsule and expanded bed integrated technology
  • Method for constructing high-efficiency bio-artificial liver system by using microcapsule and expanded bed integrated technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Preparation of liver seed cells

[0020] Primary hepatocytes were obtained from liver tissue by perfusion. Or use the hepatoma cell line HepG2, or use other immortalized hepatocytes as liver seed cells, and apply roller bottle culture to obtain sufficient amount of hepatocytes. For example, inoculate the cells in a 5L spinner bottle with 300ml of serum-containing DMEM medium, culture at 37°C and 5% carbon dioxide, and replace the medium for two to three days.

[0021] Preparation of Microcapsules of Hepatic Seed Cells Embedded with Microparticles

[0022] The medium particles are silica gel, Fe 2 o 3 Equal particles, particle size 5 nanometers to 50 microns, 1.9% sodium alginate solution 0.22 micron membrane filter sterilization, liver cells and 1.9% sodium alginate suspension, cell density 1×10 7 cells / ml, use a microcapsule generator or a high-voltage electrostatic preparation device to drop into the calcium chloride solution, and the formed calcium alginate beads...

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Abstract

The invention provides a method for constructing a high-efficiency bio-artificial liver system. The operating mode is that blood or plasma is pumped from a bed bottom by virtue of a well-designed liquid distributor, microcapsules in a bed are expanded upwards in different degrees, and when the settling speed of microcapsule granules is the same as upward flow velocity of fluid, the expanded bed is balanced, that is, the microcapsules are uniformly, stably and loosely distributed in the bed, the back-mixing degree of the liquid is extremely low, and the blood or plasma can smoothly pass through the microcapsule bed. The technology can provide a full liquid flow and material interchange environment, contributes to activity maintenance and detoxification function exerting of liver seed cells coated in the microcapsules and can obviously enhance the material interchange between the liver seed cells and blood and the material metabolism efficiency of the seed cells.

Description

technical field [0001] The invention relates to the technical field of biomedical equipment, and relates to an artificial liver system for the clinical treatment of acute liver failure, that is, the development of a high-efficiency bioartificial liver system, that is, a method for constructing a high-efficiency bioartificial liver system using microcapsule and expanded bed integration technology. Background technique [0002] Liver transplantation is the most effective treatment for end-stage liver disease. Partial hepatectomy has become a common treatment for liver tumors over the past few decades. After liver transplantation or partial hepatectomy, some patients develop postoperative liver failure due to insufficient residual liver volume, leading to death. [0003] An artificial liver is a device that replaces the function of the liver in patients with liver failure through extracorporeal circulation. Artificial liver devices include non-biological artificial liver and ...

Claims

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Application Information

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IPC IPC(8): A61M1/16C12M3/00
CPCA61M1/16C12M21/08C12M25/20
Inventor 陈卫东王艳东叶文凌徐晓周云王群
Owner HENAN UNIVERSITY
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