The invention provides a bio-organic fertilizer mixing fermentation tank, and relates to the technical field of organic fertilizer processing. The bio-organic fertilizer mixing fermentation tank comprises a machine shell, a crushing device, a conveying pipe and supporting rod, a mesh plate cover, a pressurizing water tank, a sealing cover, a connecting rod, a rotary handle, a supporting seat, a first driving motor, a second driving motor, an oxygen supply mechanism, a uniform mixing mechanism, a gear, a fermentation tank, a sealing connecting pipe, a hose, a base support, a suction air pump, adischarge hole, a sealing observation hole, a temperature and humidity sensing device, and fixed rods. The left side of the top end of the machine shell is fixedly connected with the crushing device,the right side of the top of the machine shell is fixedly connected with the pressurizing water tank, the fermentation tank is arranged in the machine shell, the oxygen supply mechanism and the uniform mixing mechanism are arranged in the fermentation tank, the bottom end of the machine shell is fixedly connected with the base support, and the bottom end of the machine shell is connected with a hose, so that the problems of fertilizer caking and serious rotting odor caused by fertilizer overturning and non-uniform oxygen supply in the biological organic fertilizer fermentation tank are solved.