Calibration method of fluorescence detection instrument

A technology of fluorescence detection and calibration method, applied in instruments, scientific instruments, fluorescence/phosphorescence, etc., can solve problems such as the inability to guarantee machine consistency, and achieve the effect of improving consistency

Pending Publication Date: 2016-09-07
深圳市华科瑞科技有限公司
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Problems solved by technology

[0005] This solution cannot guarantee the consistency of the machine when a large number of instruments are produced, and can only guarantee a rough

Method used

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  • Calibration method of fluorescence detection instrument
  • Calibration method of fluorescence detection instrument
  • Calibration method of fluorescence detection instrument

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Embodiment Construction

[0019] Such as figure 1 Shown is a diagram of a calibration principle (optical path) of the fluorescence detection instrument 5 . In the actual test, there is a fluorescent substance on the test strip 2, and the light source 1 of l1 is used to irradiate the test strip 2 to stimulate the fluorescence on the test strip 2, and through the filter 3, only the fluorescence of l2 can enter into the Signal detection module 4. In the prior art, a fixed content of fluorescent substance is used as the default standard (calibration) and placed on the fluorescence instrument 5 for measurement. The fluorescence emitted by the fluorescent substance will attenuate (the fluorescent substance will change with the change of time or light), and the use of fluorescent substance as a standard has uncertainty, and the performance of the calibrated machine will be different. Of course, there are other types of fluorometer light paths in the prior art, but they are essentially excited by a light sou...

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Abstract

The invention discloses a calibration method of a fluorescence detection instrument. The method is used for calibration of fluorescence detection instrument before leaving the factory. The light intensity detection of the fluorescence detection instrument and calibration of signal detection module parameters are carried out into two steps independently. The light intensity detection uses a light intensity measurement instrument; and the calibration of the signal detection module parameters uses a standard light source as a detection light source. The invention adopts the existing standard equipment after measurement determination for the calibration, has standard traceability and uncertainty, and is suitable for mass and accurate measurement. At the same time, the method of the invention can also be used in quality control of instruments to detect light source attenuation after long time operation; if attenuation exists, the light source can be replaced. The invention improves the consistency of fluorescence detection instrument in mass production.

Description

technical field [0001] The invention relates to a fluorescence detection instrument in the field of biomedicine, in particular to a calibration method for a fluorescence detection instrument. Background technique [0002] The characteristics of fluorescent substances are: when the fluorescent substance is irradiated by incident light of a certain wavelength, it enters an excited state after absorbing light energy, and immediately de-excites and emits outgoing light of a wavelength different from that of the incident light; and once the incident light is stopped, the phenomenon of luminescence It also disappeared immediately. The outgoing light with this property is called fluorescence. [0003] Such as figure 1 As shown, the fluorescence detection instrument (fluorescence instrument) includes a light source 1, an optical filter 3, a signal detection module 4 and a mirror group. In general, the fluorescent substance emits light of a second wavelength (called emitted light)...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2201/061
Inventor 丁卫东
Owner 深圳市华科瑞科技有限公司
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