Gas chromatography-quadrupole time-of-flight mass spectrometry/flame ionization detection method of flavor components in cigarette mainstream smoke

Abstract

A gas chromatography-quadrupole time-of-flight mass spectrometry / flame ionization detection method of flavor components in cigarette mainstream smoke comprises the following steps: collecting the flavor components in the cigarette mainstream smoke through adopting a Cambridge filter, extracting the flavor components with dichloromethane or n-hexane, taking the obtained extract liquid, analyzing the extract liquid, and allowing the obtained sample to undergo chromatographic column separation and to enter a quadrupole time-of-flight mass spectrometer (QTOF MS) and a flame ionization detector (FID) for determination in order to realize accurate qualitative diagnosis and quantification of the flavor components in cigarette mainstream smoke. The gas chromatography-quadrupole time-of-flight mass spectrometry / flame ionization detection method is applied to determination of the flavor components in cigarette mainstream smoke for the first time, and a QTOF MS spectrogram and an FID spectrogram can be simultaneously obtained only through one-shot sample introduction; and the components of a complex sample can be accurately characterized through the QTOF MS, a sample with very wide concentration range can be accurately quantified, and the retention times of the two spectrograms are completely same, so accurate qualitative diagnosis and quantification of the flavor components in cigarette mainstream smoke can be realized.

Description

technical field [0001] The invention belongs to the technical field of measuring aroma components in mainstream smoke of cigarettes, and in particular relates to a method for measuring aroma components in mainstream smoke of cigarettes. In this method, the Cambridge filter is used to capture the aroma components in the mainstream smoke of cigarettes, and then the Cambridge filter is extracted with solvents such as dichloromethane or n-hexane, and the extract is analyzed. After the sample is separated by the chromatographic column, it enters the quadrupole flight Time-to-time mass spectrometer (QTOF MS) and flame ionization detector (FID) were used to determine the accurate qualitative and quantitative analysis of aroma components in mainstream cigarette smoke. Background technique [0002] The aroma components in the mainstream smoke of cigarettes are one of the important indicators for evaluating the quality of tobacco and cigarettes. Clarifying the composition of key arom...

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Problems solved by technology

[0005] At present, two instruments are usually used for the above analysis, that is, a GC-QTOF MS instrument and a gas chromatograph equipped with an FID, or a chromatograph equipped with an FID coupled with a QTOF MS instrument. Among them, the chromatograph is equipped with two injection ports and connected to two columns. The above analysis methods all have the problem of inconsistency in the retention time of the mass spectrum signal and the FID signal of the same substance, which affects the accurate analysis.

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