The invention discloses a cooling oil content measuring method. The cooling oil content measuring method comprises a step of qualitative and quantitative analysis on one or more ingredients of menthol, camphor, eucalyptol and eugenol. The method comprises the following specific steps: (1) taking the menthol, the camphor, the eucalyptol and the eugenol as reference objects; (2) treating a cooling oil sample by using a solvent, and then detecting the cooling oil sample by using a GC (Gas Chromatography) method; (3) a GC measuring method is as follows: a chromatographic column and a detector are used, wherein the chromatographic column is an HP-INNO WAX capillary column; the detector is a flame ionization detector; the temperature of the detector is 250-300 DEG C, the temperature of a sample inlet is 220-270 DEG C, the temperature of the column is increased in a programmed manner from an initial temperature of 60-110 DEG C to 220-280 DEG C, the temperature is increased by 5-15 DEG C every minute and kept for 8-20 minutes; sampling mode: split sampling is adopted, and the split ratio is 20:1. The method is scientific, reasonable, simple, convenient, easy to implement and suitable for quality control of volatile components including the menthol, the camphor and the like of the cooling oil and relevant extractives.