Gas chromatography-quadrupole time-of-flight mass spectrometry/flame ionization detection method for tobacco essence perfume

Abstract

Disclosed is a gas chromatography-quadrupole time-of-flight mass spectrometry/ flame ionization detection method for tobacco essence perfume. The method includes subjecting the tobacco essence perfume to organic solvent extraction and centrifugation, taking an organic solvent layer to analyze, regulating tail-end distribution of a chromatographic column through a capillary flow technology after the sample is subjected to chromatographic column separation, and determining by a QTOF MS (quadrupole time-of-flight mass spectrometer) and FID (flame ionization detector) to achieve accurate qualitativeness and quantitativeness of the tobacco essence perfume. The gas chromatography-quadrupole time-of-flight mass spectrometry/ flame ionization detection method is put forward for the first time and is applied to determination of the tobacco essence perfume, QTOF MS and FID spectrograms can be obtained simultaneously only by one-needle sample injection, QTOF MS can determine components of complicated samples accurately, the FID is capable of qualifying the sample with wide concentration range accurately, and the retention time of the QTOF MS spectrogram and the retention time of the FID spectrogram are the same, so that accurate qualitativeness and quantitativeness of complicated tobacco essence perfume samples can be achieved.

Description

technical field [0001] The invention belongs to the technical field of measuring flavors and fragrances for tobacco, and in particular relates to a method for measuring flavors and fragrances for tobacco. Background technique [0002] Tobacco flavors and fragrances are often used to regulate cigarette products with different styles and characteristics. They also play an important role in improving the taste of cigarettes, compensating and enhancing the aroma of cigarettes, and most of them are derived from natural flavors and fragrances. Due to different raw materials and production processes, different tobacco flavors and fragrances have large differences in composition. It is difficult to accurately understand the flavors and fragrances simply by conventional indicators such as acidity, refractive index, miscibility, and total volatile matter and the experience of the perfumer. ingredients. At present, gas chromatography-mass spectrometry (GC-MS) or gas chromatography (GC...

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Problems solved by technology

[0004] At present, two instruments are usually used for the above analysis, that is, a GC-QTOF MS instrument and a gas chromatograph equipped with an FID, or a chromatograph equipped with an FID coupled with a QTOF MS instrument. Among them, the chromatograph is equipped with two injection ports and connected with two columns. The above analysis methods all have the problem of inconsistency in the retention time of the mass spectrum signal and FID signal of the same substance, which affects the accurate analysis.

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