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Primer, probe and kit for evaluating skin anti-allergy condition

An anti-allergy and kit technology, applied in the field of beauty, can solve problems such as difficult to achieve personalized maintenance or treatment, and achieve the effect of clear molecular mechanism, high sensitivity and strong specificity

Inactive Publication Date: 2016-09-21
FUJIAN AIWO HEALTH BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above patents / patent applications all use foreign substances to regulate the expression of genes in the body, but there are different SNP sites in the genes of each individual. This method is difficult to achieve personalized maintenance or treatment

Method used

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  • Primer, probe and kit for evaluating skin anti-allergy condition
  • Primer, probe and kit for evaluating skin anti-allergy condition
  • Primer, probe and kit for evaluating skin anti-allergy condition

Examples

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Embodiment 1

[0108] Example 1: Primer and probe design

[0109] 1. Design and synthesize the following primers and probes. Synthesis unit: Applied Biosystems, USA Applied Biotechnology Co., Ltd.

[0110] TMEM232 (rs7701890) primer and fluorescent probe sequence:

[0111] Forward primer TGTTGGCTTTAATTGTTCAGA SEQ ID NO: 1;

[0112] Reverse primer TGCCACAGAAATATAAGGATCA SEQ ID NO: 2;

[0113] Fluorescent Probe FAM-ATTACTATAT A ATTTAGACTTG-MGB SEQ ID NO: 3;

[0114] Fluorescent probe VIC-ATTACTATAT G ATTTAGACTTG–MGB SEQ ID NO: 4;

[0115] TMEM232 (rs10067777) primer and fluorescent probe sequence:

[0116] Forward primer ACCTTTTCAAAACACAAGCCT SEQ ID NO: 5;

[0117] Reverse primer GCCTCTATGCTTATGTCAGTCC SEQ ID NO: 6;

[0118] Fluorescent Probe FAM-AGACTCTAC A CTTCTTTAAT-MGB SEQ ID NO: 7;

[0119] Fluorescent probe VIC-AGACTCTAC G CTTCTTTAAT-MGB SEQ ID NO: 8;

[0120] RTEL1-TNFRSF6B (rs6010620) primer and fluorescent probe sequence:

[0121] Forward primer CCAGCCTAGAGCTAGAAACGG SEQ ...

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PUM

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Abstract

The invention discloses a primer, a probe and a kit for evaluating skin anti-allergy condition. The sequences of the primer and the probe are SEQ ID NO:1-4, SEQ ID NO:5-8, SEQ ID NO:9-12, SEQ ID NO:13-16, SEQ ID NO:17-20, SEQ ID NO:21-24, SEQ ID NO:25-28, SEQ ID NO:29-32, and SEQ ID NO:33-36, wherein the 5' terminals and 3'terminals of SEQ ID NO:3-4, 7-8, 11-12, 15-16, 19-20, 23-24, 27-28, 31-32 and 35-36 are respectively connected with different fluorescent markers. The invention also discloses a method using gene detection to assist a customer to select correct face-beautifying methods. The primer and the probe have the advantage that by acknowledging the internal factors and external factors of skin allergy, a personalized face-beautifying and health solution is provided, and the effect of an anti-allergy face-beautifying product is maximized.

Description

technical field [0001] The invention relates to the cosmetic field, in particular to a primer, a probe and a kit for evaluating skin anti-allergic conditions. Background technique [0002] SNP (single nucleotide polymorphism) refers to the variation of a single nucleotide on the genome, including transitions, transversions, deletions, and insertions. It is the most common genetic marker, and its carrying frequency in the population is greater than 1%. , and are widely used in the fields of human disease diagnosis, individualized drug use, and basic biological research. With the in-depth study of different SNP sites, through the combined detection of multiple SNP sites and subsequent data analysis, personalized health solutions can be formulated for the genetic information of different groups of people. [0003] The appearance of human skin is affected by internal and external factors. External factors include ultraviolet rays, environmental pollution, improper use of beauty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6883C12Q2600/156C12Q2563/107C12Q2561/101
Inventor 纪志梁王毛阳杨志云刘晓瑜
Owner FUJIAN AIWO HEALTH BIOTECH CO LTD
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