Breeding method of hybrid Bama miniature pigs
A technology of Bama fragrant pig and breeding method, which is applied in the field of animal husbandry and breeding, can solve the problems of inability to meet the standard of reference water, low treatment efficiency of treatment technology, and less treatment of feces and sewage, achieve simple and feasible breeding mode, and reduce breeding costs. , the effect of high litter survival rate
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Embodiment 1
[0038] 1. Preparation of Crosslinked Chitosan Microspheres
[0039] Dissolve 5g chitosan in 100mL mass fraction of 2% acetic acid solution, stir for 10 minutes, then put it into a three-necked flask, add 50mL deionized water and 2mL mass fraction of 25% glutaraldehyde solution, put the three-necked flask into a water bath at 50°C, and carry out the cross-linking reaction for 4 hours while stirring, wash the cross-linked chitosan gel with deionized water until neutral, dry it, add it to a three-necked flask, add 60 mL of deionized water and Put 10mL of epichlorohydrin solution with a mass fraction of 20%, put the three-neck flask into a 50°C constant temperature water bath, and react for 5 hours. After the reaction, let it cool naturally, filter with suction, and wash the filter residue with acetone and deionized water in sequence To neutrality, dry at 50°C for 24 hours to obtain cross-linked chitosan microspheres.
[0040] 2. a breeding method for hybrid Bama fragrant pigs, c...
Embodiment 2
[0048] 1. Preparation of Crosslinked Chitosan Microspheres
[0049] Dissolve 6g chitosan in 110mL mass fraction of 3% acetic acid solution, stir for 12 minutes, then put it into a three-necked flask, add 50mL deionized water and 2mL mass fraction of 26% glutaraldehyde solution, put the three-necked flask Put it into a water bath at 52°C, carry out the cross-linking reaction for 2 hours while stirring, wash the cross-linked chitosan gel with deionized water until neutral, dry it, add it to a three-necked flask, add 60 mL of deionized water and 10mL of epichlorohydrin solution with a mass fraction of 22%, put the three-necked flask into a 55°C constant temperature water bath, and react for 3 hours. After the reaction, let it cool naturally, filter with suction, and wash the filter residue with acetone and deionized water in sequence To neutrality, dry at 55°C for 20 hours to obtain cross-linked chitosan microspheres.
[0050] 2. a breeding method for hybrid Bama fragrant pigs, ...
Embodiment 3
[0058] 1. Preparation of Crosslinked Chitosan Microspheres
[0059] Dissolve 7g chitosan in 120mL mass fraction of 4% acetic acid solution, stir for 12 minutes, then put it into a three-necked flask, add 50mL deionized water and 2mL mass fraction of 28% glutaraldehyde solution, put the three-necked flask into a water bath at 58°C, and carry out the cross-linking reaction for 3 hours while stirring, wash the cross-linked chitosan gel with deionized water until neutral, dry it, add it to a three-necked flask, add 60 mL of deionized water and 10mL of epichlorohydrin solution with a mass fraction of 27%, put the three-necked flask into a 54°C constant temperature water bath, and react for 4 hours. After the reaction, let it cool naturally, filter with suction, and wash the filter residue with acetone and deionized water in sequence To neutrality, dry at 57°C for 20 hours to obtain cross-linked chitosan microspheres.
[0060] 2. a breeding method for hybrid Bama fragrant pigs, com...
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