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Resuscitation fluid applied to first aid of hemorrhagic shock of pets and preparation method thereof

A technology for resuscitating fluids and pets, which is applied in the direction of blood diseases, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of little effect of expanding blood volume and single components, and avoid the spread of related infectious diseases. Preparation method Simple, blood volume expansion effect

Pending Publication Date: 2016-12-21
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the existing blood substitutes have a single component, only a single oxygen supply, and have little effect on expanding blood volume, and provide a resuscitation fluid for first aid in pet hemorrhagic shock and its preparation method

Method used

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  • Resuscitation fluid applied to first aid of hemorrhagic shock of pets and preparation method thereof
  • Resuscitation fluid applied to first aid of hemorrhagic shock of pets and preparation method thereof
  • Resuscitation fluid applied to first aid of hemorrhagic shock of pets and preparation method thereof

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preparation example Construction

[0024] The present invention firstly provides a method for preparing resuscitation fluid applied to pets in first aid of hemorrhagic shock, the method comprising:

[0025] Step 1: react bovine hemoglobin solution with glutaraldehyde solution, and centrifuge to obtain hemoglobin particles cross-linked by glutaraldehyde;

[0026] Step 2: Mix the glutaraldehyde-crosslinked hemoglobin particles obtained in Step 1 with the injection to obtain a resuscitation solution for first aid in pet hemorrhagic shock;

[0027] The injection is HES, HES2000 or physiological saline.

[0028] According to the present invention, bovine hemoglobin solution and glutaraldehyde solution are firstly prepared respectively, the concentration of the bovine hemoglobin solution is preferably 1-40mg / ml; the concentration of the glutaraldehyde solution is preferably 0.025-0.25%.

[0029] According to the present invention, the above prepared bovine hemoglobin solution and glutaraldehyde solution are mixed fo...

Embodiment 1

[0037] Bovine hemoglobin solution (concentration: 30mg / ml) and glutaraldehyde solution (concentration: 0.01%) were mixed according to the molar ratio of 4:1, and reacted at room temperature for 12h, and the reactant was centrifuged at 2000rpm for 10min to obtain glutaraldehyde Cross-linked hemoglobin particles;

[0038] Mix glutaraldehyde-crosslinked hemoglobin particles with HES at a mass ratio of 1:4, convert the prepared solution to a deoxidized state under the protection of carbon monoxide, filter and sterilize after conversion, and store it under normal temperature and vacuum conditions. Resuscitative solution for first aid in pet hemorrhagic shock.

[0039] figure 1 It is the gas conversion capacity diagram of the resuscitation fluid in Example 1 of the present invention, from figure 1 It can be seen that the resuscitation fluid can carry oxygen and release oxygen, and has the ability to combine and release oxygen.

[0040] The resuscitation fluid prepared in Example ...

Embodiment 2

[0042] Bovine hemoglobin solution (concentration: 30mg / ml) and glutaraldehyde solution (concentration: 0.01%) were mixed according to the molar ratio of 4:1, and reacted at room temperature for 12h, and the reactant was centrifuged at 2000rpm for 10min to obtain glutaraldehyde Cross-linked hemoglobin particles;

[0043] Mix glutaraldehyde-crosslinked hemoglobin particles with HES2000 at a mass ratio of 1:4, convert the prepared solution to a deoxygenated state under the protection of carbon monoxide, filter and sterilize after conversion, and store it under normal temperature and vacuum conditions. Resuscitative fluid for first aid in pet hemorrhagic shock.

[0044]The resuscitation solution prepared in Example 2 was carried out to the shock rats. After the resuscitation solution of the present invention was input, the mean arterial pressure (MP) dropped to the extreme value after bloodletting, and the blood pressure recovered with the liquid infusion, and the blood pressure i...

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Abstract

The invention provides a resuscitation fluid applied to the first aid of hemorrhagic shock of pets and a preparation method thereof, belongs to the field of a preparation method of resuscitation fluid, and solves problems that the conventional blood substitutes are single in component, only supply single oxygen and have fewer effects on the expanding of blood volume. The preparation method comprises the following steps: firstly, carrying out a reaction between bovine hemoglobin solution and glutaraldehyde solution, and centrifuging to obtain hemoglobin particles cross-linked with glutaraldehyde; and then, mixing the obtained hemoglobin particles cross-linked with glutaraldehyde with injection solution, to obtain the resuscitation fluid applied to the first aid of hemorrhagic shock of pets, wherein the injection solution is HES, HES2000 or physiological saline. The invention further provides the resuscitation fluid obtained according to the preparation method. The preparation method provided by the invention is simple; the raw materials are easy to obtain; the prepared resuscitation fluid can reversibly bind and release oxygen in vivo and simultaneously expand the blood volume.

Description

technical field [0001] The invention belongs to the field of preparation methods of resuscitation fluid, and in particular relates to a resuscitation fluid applied to pets for first aid in hemorrhagic shock and a preparation method thereof. Background technique [0002] Blood transfusion is a safe and effective life-saving therapy to replenish an animal's blood or a component. The blood of adult dogs is 80-90ml / kg, and the blood of cats is 80-90ml / kg. When the acute blood loss reaches 1 / 5 of the whole blood volume due to trauma or operation, blood pressure will drop and the heartbeat will speed up. When the blood loss reaches 1 / 3, it will be life-threatening. At this time, timely blood transfusion is the most effective way to save lives. Blood transfusion can not only supplement the blood volume, but also the increased red blood cells can increase the oxygen carrying capacity and improve the function of the heart muscle and brain. At present, it is generally recognized int...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/42A61K9/08A61P7/08
CPCA61K38/42A61K9/0026A61K9/08
Inventor 黄宇彬齐延新谢志刚景遐斌
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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