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Magnetic nanometer material and application thereof to phosphopeptide enrichment

A magnetic nanometer and reaction technology, applied in the field of analytical chemistry, can solve the problems of loss of phosphorylated peptides and waste of time, and achieve the effect of high selectivity, high enrichment specificity and selectivity of phosphorylated peptides

Inactive Publication Date: 2017-01-04
BEIJING PROTEOME RES CENT
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Problems solved by technology

But conventional TiO 2 During the enrichment process, the material needs more eluent to elute the material, and then the phosphopeptides are collected by centrifugation, and the centrifugation process is a waste of time and easily causes the loss of phosphorylated peptides

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  • Magnetic nanometer material and application thereof to phosphopeptide enrichment
  • Magnetic nanometer material and application thereof to phosphopeptide enrichment

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Embodiment Construction

[0018] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0019] see Figure 1-2 , a magnetic nanomaterial and its use in phosphopeptide enrichment, comprising:

[0020] (1) Preparation of magnetic nanomaterials loaded with mixed lanthanide metals

[0021] NH 2 -MNPs as Fe 3 o 4 @TCPP-DOTA-M 3+ The precursors were synthesized by first combining 4-carboxyphenylporphyrin (TCPP) and 1,4,7,10-tetraazacyclododecane-N,N,N,N-tetraacetic acid (DOTA ) to covalently modify the surface of the material, and then a variety of lanthanide metal ions are immobilized on the surface of magnetic nanomaterials through chelation with DOTA.

[0022] The specific operation process is as follows: (1) Weigh 10mg TCPP and dissolve it in 10mL ethanol solution, and sonicate for 30min. Add EDC / NHS at a ratio of 10:3 to activate part of the carboxyl groups of TCPP in the reaction vessel. After reacting at room temperature for 1...

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Abstract

The invention discloses a magnetic nanometer material and application thereof to phosphopeptide enrichment. 4-carboxyphenyl porphyrin (TCPP) and 1,4,7,10-tetraazacycl ododecane-N,N,N,N-tetraacetate (DOTA) are in covalent linkage to the surface of an amino-modified magnetic nanometer material modified through ethanediamine, and lanthanide series metal ions are immobilized on the surface of the material to synthesize a novel magnetic nanometer material. The metal ion-modified magnetic nanometer material has higher selectivity, sensitivity and enrichment efficiency in the phosphopeptide enrichment process. Compared with the traditional TiO2 method, the metal ion-modified magnetic nanometer material has higher phosphopeptide enrichment specificity and selectivity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a magnetic nanometer material and its application in phosphopeptide enrichment. Background technique [0002] Phosphorylated proteins are derived from dynamic and reversible protein phosphorylation modification processes, which are involved in almost all physiological and biochemical processes, including apoptosis, signal transduction, transcriptional regulation, translational regulation, etc. Phosphoproteomics has a very close relationship with people's cognition of life activities and the diagnosis and treatment of major diseases, and has always been a research hotspot. Abnormal protein phosphorylation process is associated with the occurrence and development of some serious diseases. In recent years, with the rapid development of biological mass spectrometry technology, high-sensitivity mass spectrometers have become an important tool for phosphoproteomics research. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/28B01J20/30H01F1/36C07K1/20G01N30/08G01N30/89
CPCB01J20/223B01J20/28009C07K1/20G01N30/08G01N30/89H01F1/0054H01F1/344H01F1/36
Inventor 钱小红秦伟捷张万军
Owner BEIJING PROTEOME RES CENT
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