The invention discloses an online automatic analysis device and an analysis method for phosphorylated proteomics. The device is a liquid chromatograph-mass spectrometer, the liquid chromatograph comprises a phosphorylated peptide capture column and an analytical column, and the phosphorylated peptide capture column is an ATP modified immobilized metal ion affinity chromatographic column; and potassium water glass, gamma-glycidyl ether oxypropyl trimethoxy silane and water-dissolved adenosine disodium triphosphate are mixed and stirred, then formamide dissolved with water is added and stirred to obtain a reaction solution, a chromatographic column is filled with the reaction solution, then the reaction solution filling the chromatographic column is subjected to a reaction curing, washing is performed, and the ATP modified immobilized metal ion affinity chromatographic column is obtained. The device has the primary advantage that researchers are liberated from complicated manual operations such as metal ion loading, sample loading, washing, elution, desalting, drying and redissolving required by enrichment of phosphorylated peptides, so that the experiment efficiency is improved.