An online automated analysis device and analysis method for phosphoproteomics

A phosphorylated protein, automated analysis technology, applied in the fields of chromatography and mass spectrometry, can solve the problems of high back pressure of phosphorylated peptide enrichment column, incompatible loading conditions, poor sensitivity, etc. The effect of improving consistency
CN113607868BActive Publication Date: 2022-03-15AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
Publication Date
2022-03-15

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Abstract

The invention discloses an online automatic analysis device and analysis method for phosphorylated proteomics. It is a liquid chromatography-mass spectrometer, and the liquid chromatography includes a phosphorylated peptide capture column and an analysis column, and the phosphorylated peptide capture column is an ATP-modified immobilized metal ion affinity chromatography column; the potassium Mix and stir water glass, γ-glycidyl etheroxypropyl trimethoxysilane and water-dissolved adenosine triphosphate disodium, then add formamide dissolved in water and stir to obtain a reaction solution, fill the reaction solution with the chromatographic column, and then fill the chromatographic column The reaction solution of the column is solidified by reaction, and then washed to obtain an ATP-modified immobilized metal ion affinity chromatography column. The primary advantage of the present invention is that researchers are freed from complex manual operations such as metal ion loading, sample loading, washing, elution, desalination, evaporation, and reconstitution required for enrichment of phosphorylated peptides, which improves experimental efficiency .
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Description

technical field

[0001] The invention belongs to the field of chromatography and mass spectrometry, and in particular relates to an online automatic analysis device and analysis method for phosphorylated proteomics, which perform automatic online analysis of phosphorylated polypeptides on a nanoliter liquid chromatography-mass spectrometer. Background technique

[0002] Many important life regulation processes are related to protein phosphorylation. Qualitative and quantitative analysis of protein phosphorylation events in cells will help reveal the regulatory mechanism of phosphorylated modified proteins in signaling pathways, mine key genes or guide drug development. target. Liquid chromatography-high resolution mass spectrometry combined with bottom-up analysis strategy is the core method of current proteomics research. However, due to the extremely low abundance of phosphorylated peptides, poor ionization efficiency, and signal inhibition by non-phosphorylated peptides, ...

Claims

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