Preparation of a kind of organic monolithic small column and organic monolithic small column and application

A holistic and organic technology, applied in solid adsorbent liquid separation, chemical instruments and methods, separation methods, etc., can solve the problem that centrifugal enrichment cartridges are not used, ZipTip cartridges are expensive, and polydopamine lacks chemical stability, etc. problem, to achieve the effect of enrichment ability, short time-consuming and rapid analysis
CN107297086BInactive Publication Date: 2019-04-23DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2019-04-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the preparation of a Ti(IV)-IMAC organic monolithic small column, which is used for phosphorylated proteomics analysis of trace biological samples. The organic monolithic column material is a polymethacrylate polymer with rich pore structure, high specific surface area, and many active sites for enriching titanium ions; at the same time, due to its hydrophilic material surface, Phosphopeptide enrichment showed a strong enrichment ability. It was used in the phosphoproteomics analysis of 5g HeLa cells, and more than 1000 non-redundant phosphorylation sites were identified on average, and the enrichment specificity was as high as 92.5%. More importantly, the preparation of the Ti(IV)-IMAC organic monolithic column is simple. Compared with the packed column, it can be prepared by light-induced free radical polymerization in only a few minutes; and there is no need to prepare a stopper, The preparation time of the enrichment material is reduced to a large extent.
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Description

technical field

[0001] The invention belongs to the technical field of phosphoproteomics in the direction of proteomics research, and specifically relates to a sample processing method applied to phosphoproteomics. Background technique

[0002] As an important protein post-translational modification, protein phosphorylation plays a crucial role in the regulation of cellular metabolism. Abnormal phosphorylation modifications are closely related to many disease processes, such as malignant tumors. At present, the proteomic analysis strategy based on the shotgun method is the main method in the study of phosphoproteomics. In this method, protein samples are first digested into peptides, enriched by phosphopeptides, and analyzed by LC-MS / MS. Due to the high dynamic range of phosphorylation modification, the low abundance of phosphorylated proteins, and the poor ionization effect of phosphorylated peptides, the key step that affects the accuracy and sensitivity of the entire ph...

Claims

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