Method for purifying tyrosine phosphopeptide

A technology of tyrosine phosphorylation and tyrosine phosphopeptide, which is applied in the purification field of tyrosine phosphorylated peptide, can solve the problems of harsh solvent and temperature requirements, limited reagent price, difficult protein identification, etc., and achieves high sensitivity, Highly specific and selective effects
CN102199189AInactive Publication Date: 2011-09-28DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2011-09-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses application of a beta-elimination and Michael nucleophilic addition method in study of phosphoproteomics, in particular a method for purifying tyrosine phosphopeptide. The method comprises the following steps of: eliminating phosphate modification groups on non-tyrosine phosphopeptide in protein hydrolysate by using beta-elimination and Michael nucleophilic addition; and enriching the tyrosine phosphopeptide which is not subjected to the elimination reaction by using a phosphoeptide enriching material. By utilizing the high specificity of the beta-elimination and Michael nucleophilic addition, the tyrosine phosphopeptide is purified from a complicated protein hydrolysate sample in high selectivity.
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Description

technical field

[0001] The present invention is a purification method of tyrosine phosphorylated peptide based on β-elimination and Michael nucleophilic addition reaction, and is the application of β-elimination and Michael nucleophilic addition method in phosphorylated proteome, β-elimination The method of nucleophilic addition reaction with Michael shows high specificity, high selectivity and high sensitivity to tyrosine phosphorylated peptides. β-elimination and Michael nucleophilic addition reactions can realize the purification of tyrosine phosphopeptides, which can be applied to the phosphoproteome of protein post-translational modification. Eliminate the phosphate groups on serine and threonine by β-elimination and Michael nucleophilic addition reaction methods, and then use phosphopeptide enrichment materials to purify tyrosine phosphorylated peptides from complex proteolysis samples, combined with biological mass spectrometry Perform large-scale identification of tyr...

Claims

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