Preparation method of nano calcium phosphate for polyphosphopeptide enrichment and phosphorylation site identification as well as product and application of nano calcium phosphate

A nano-calcium phosphate and phosphorylation technology, applied in the preparation of test samples, chemical instruments and methods, phosphorus compounds, etc., can solve problems such as in-depth research on the biological functions of unfavorable phosphorylated proteins, and achieve broad application prospects and preparation methods Simple, Inexpensive Effects
CN112499607AActive Publication Date: 2021-03-16NANJING NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING NORMAL UNIVERSITY
Publication Date
2021-03-16

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Abstract

The invention discloses a preparation method of nano calcium phosphate for polyphosphopeptide enrichment and phosphorylation site identification as well as a product and application of the nano calcium phosphate, and the preparation method comprises the following steps: sequentially adding a calcium salt aqueous solution and a phosphate aqueous solution into a slightly alkaline solution, conducting stirring, and conducting drying after the reaction is finished, so as to obtain a nano calcium phosphate material. Due to strong interaction between calcium ions and phosphate radicals, nano calciumphosphate can effectively realize selective enrichment of polyphosphopeptides and identification of phosphorylation sites. Meanwhile, the calcium phosphate nano material prepared by the method is a biological functional material which is simple to synthesize, low in cost and high in stability, and has a wide application prospect in phosphorylated proteomics research.
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Description

technical field

[0001] The invention relates to a material for selectively enriching polyphosphopeptides, in particular to a method for preparing nano-calcium phosphate used for enriching polyphosphopeptides and identifying phosphorylation sites, as well as its products and applications. Background technique

[0002] Protein phosphorylation is one of the most important post-translational modifications in organisms, regulating many important processes in life activities, including cell proliferation, differentiation, apoptosis and metabolism. Protein phosphorylation includes not only single phosphorylation modification but also multiple phosphorylation modification, and the identification of multiple phosphorylation modification and its site has a more significant impact on the biological activity of the protein. For example, tubulin Tau in the adult brain is an important polyphosphorylated protein, and its longest isoform (Tau441) contains a total of 80 serine (Ser) and thre...

Claims

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