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Method for identifying existence form biomarker spectrum of human growth hormone protein

A biomarker, growth hormone technology, applied in the field of biomarker spectrum identification of the form of human growth hormone protein, can solve the problem of unsatisfactory clinical treatment effect

Pending Publication Date: 2021-06-01
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are many treatment methods and methods such as drug treatment and surgical resection, the clinical treatment effect of a series of GH-related diseases caused by pituitary adenoma is still unsatisfactory.

Method used

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  • Method for identifying existence form biomarker spectrum of human growth hormone protein
  • Method for identifying existence form biomarker spectrum of human growth hormone protein
  • Method for identifying existence form biomarker spectrum of human growth hormone protein

Examples

Experimental program
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Embodiment 1

[0027] The method for identifying the biomarker profile of human growth hormone protein in the present embodiment, the method is:

[0028] S1. Collect human growth hormone-secreting pituitary adenoma and normal pituitary tissue samples, respectively lyse the tissues and extract the two groups of tissue proteins. The specific method is:

[0029] (1), pituitary tissue samples:

[0030]All human growth hormone-secreting pituitary adenoma tissues (n=7) in this example were obtained from the Department of Neurosurgery of Xiangya Hospital, and were approved by the Medical Ethics Committee of Xiangya Hospital, Central South University. The normal pituitary tissue samples (n=7) used as controls were obtained from the following sources: 4 cases were obtained from the University of Tennessee Health Center, approved by the Ethics Institution Review Committee of the University of Tennessee Medical Research Center; 3 cases were obtained from the Department of Forensic Medicine, Tongji Medi...

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Abstract

The invention provides a method for identifying a human growth hormone protein existence form biomarker spectrum, which comprises the following steps: collecting human growth hormone secreting pituitary adenoma and normal pituitary tissue samples, respectively extracting tissue proteins, carrying out two-way gel electrophoresis, western blot and coomassie brilliant blue staining, scanning a visual PVDF membrane and a two-way gel into a digital image, digesting corresponding two-way gel protein spots with trypsin, purifying, and identifying a GHP biomarker spectrum through mass spectrum identification and bioinformatics analysis; and identifying post-translational modifications and shear variations on GHP by using quantitative phosphorylated proteomics, ubiquitinated proteomics, and acetylated proteomics analysis in combination with bioinformatics. According to the invention, the GHP change mode between the growth hormone secreting pituitary adenoma and normal pituitary tissues can be identified, 46 kinds of GHPs are identified in the growth hormone secreting pituitary adenoma, 35 kinds of GHPs are identified in the normal pituitary tissues, and 11 kinds of GHPs only appear in the growth hormone secreting pituitary adenoma tissues.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method for identifying the biomarker spectrum of the form of human growth hormone protein. Background technique [0002] Pituitary adenoma is one of the most common primary intracranial tumors, with a prevalence of 17% worldwide. Abnormal growth hormone (GH) secretion by pituitary adenomas can cause clinical disease. In childhood, excessive GH secretion leads to abnormally large bone formation gigantism, while insufficient GH secretion leads to dwarfism; in adults, pituitary Hypersecretion of GH by adenoma leads to acromegaly. Pituitary adenomas cause some GH-related diseases to affect human health for a long time. Although there are many treatment methods and methods such as drug treatment and surgical resection, the clinical treatment effect of a series of GH-related diseases caused by pituitary adenoma is still unsatisfactory. Therefore, finding grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447G01N30/02G01N30/72G01N33/68
CPCG01N27/447G01N30/02G01N30/72G01N33/6848G01N2333/61G01N33/74G01N2474/10G06T7/0014G06T2207/10024G06T2207/30024G01N1/30G01N27/44726G01N30/06G01N33/6851G01N2030/027G01N2030/062G06T7/0012G06T2207/10004G06T2207/30096
Inventor 詹显全李彪王小伟李娜
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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