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Preparation method of dental pulp stem cell and chitosan scaffold complex

A technology of dental pulp stem cells and chitosan, which is applied in the field of composite preparation, can solve problems such as defective clinical application prospects, and achieve the effect of good biocompatibility

Inactive Publication Date: 2017-02-08
AFFILIATED HOSPITAL OF NANTONG UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, these mesenchymal stem cells are clinically limited by their sources and acquisition methods, and their clinical application prospects are limited.

Method used

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  • Preparation method of dental pulp stem cell and chitosan scaffold complex
  • Preparation method of dental pulp stem cell and chitosan scaffold complex
  • Preparation method of dental pulp stem cell and chitosan scaffold complex

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Embodiment Construction

[0031] The implementation of the present invention will be illustrated by specific specific examples below, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0032] A method for preparing a composite of dental pulp stem cells and chitosan scaffolds, including the isolation and primary culture of human dental pulp stem cells, the identification of chondrogenic differentiation of dental pulp stem cells, the identification of adipogenic differentiation of dental pulp stem cells, and the preparation of porous chitosan scaffolds The preparation and co-cultivation steps complete the preparation of the dental pulp stem cells and the chitosan scaffold complex; the specific steps are as follows:

[0033] (1) Isolation and primary culture of human dental pulp stem cells: Take clinically extracted complete third molars, soak the isolated teeth in povidone iodine for 1 minute, rinse ...

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Abstract

The invention discloses a preparation method of a dental pulp stem cell and chitosan scaffold complex. The preparation of the dental pulp stem cell and chitosan scaffold complex is finished through steps of separation and primary culture of human dental pulp stem cells, chondrogenic differentiation and identification of the dental pulp stem cells, adipogenic differentiation and identification of the dental pulp stem cells, preparation of a porous chitosan scaffold and co-culture. According to the chitosan scaffold prepared by the invention, an adhesion condition between the dental pulp stem cells and chitosan is observed under an electron microscope, so as to effectively testify that the dental pulp stem cells and the chitosan have good biological compatibility; meanwhile, the chitosan scaffold has no toxic responses on the dental pulp stem cells.

Description

technical field [0001] The invention relates to a preparation method of a composite, in particular to a preparation method of a dental pulp stem cell and chitosan scaffold composite. Background technique [0002] At present, the mesenchymal stem cells used in neural tissue regeneration engineering research are mainly bone marrow mesenchymal stem cells and adipose mesenchymal stem cells from bone marrow and adipose tissue. Studies have shown that they can be directed to differentiate into SCs and have the ability to form myelin. However, these mesenchymal stem cells are clinically limited by their sources and acquisition methods, and their clinical application prospects are limited. In recent years, researchers have successfully isolated and cultured a new type of mesenchymal stem cell-dental pulp stem cell from the dental pulp tissue of adult third molars. And stem cells with tissue repair ability. Research on dental pulp stem cells is also increasing. Daniel.L et al. co...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/20A61L27/50A61L27/54
CPCA61L27/20A61L27/3834A61L27/3878A61L27/3895A61L27/50A61L27/54A61L2300/43A61L2430/32C08L5/08
Inventor 冯兴梅李立人顾志峰黄丹
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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