Optimized rice indica and japonica performance detection system and optimized rice indica and japonica performance detection method

Abstract

The invention discloses an optimized rice indica and japonica performance detection system. The optimized rice indica and japonica performance detection system comprises a molecular marker system, an amplification system, an electrophoresis system and a gel imaging system, wherein the molecular marker system is used for molecular marking of a group of indica and japonica high in specificity, the amplification system is used for PCR (polymerase chain reaction) amplification, the electrophoresis system is used for PAGE (polyacrylamide gel electrophoresis), and the gel imaging system is used for imaging detection of banding patterns. The optimized rice indica and japonica performance detection system has the advantages that the detected banding patterns are clear and obvious, and adopting a GoldView aqueous solution for staining substitutes for a traditional PAGE gel silver staining method, so that an operation process is simpler and more convenient, and the amplification efficiency and the detection efficiency are both improved. The invention further provides a rice indica and japonica performance differentiating method. A designed indica and japonica performance differentiating marker is high in specificity, only one indica or japonica specific band pattern occurs, and the occurrence frequency of the specific band pattern in an indica and japonica group is above 70.0%. The indica and japonica performance is measured through the indica and japonica degree, and accordingly comparison results are more objective and comprehensive.

Description

technical field [0001] The invention relates to a detection system, in particular to an optimized rice indica and japonica detection system and method. Background technique [0002] Asian cultivated rice (Oryza sativa L.) is divided into two subspecies: indica and japonica. The research on rice indica-japonica differentiation can be carried out from the aspects of morphological markers, biochemical markers and molecular markers. The Asian rice indica and japonica subspecies identification method established by Cheng Kansheng (Cheng Kansheng et al., 1984) is simple and easy to implement, but it is limited by factors such as growth environment and rice growth cycle. Compared with morphological and biochemical methods, since molecular markers directly reflect the heritable variation in the nucleotide sequence, it can more accurately and efficiently identify indica and japonica subspecies, and accurately reveal genetic differences. With the completion of the genome sequence de...

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Problems solved by technology

[0003] In the process of using the published indica-japonica specific markers to discriminate rice indica-japonica attributes, the following problems were found: (1) some indica-japonica markers tend to have skewed distribution in the detection population, such as in the japonica rice population There are also a large number of indica stripes ( figure 1 ); for some indica-japonica markers, the indica-japonica distinction is not high, for example, in the indica population, the frequencies of indica-type bands and japonica-type bands are almost the same ( figure 1 ); some indica and japonica markers have high polymorphisms, and there are multiple band types in the population, which do not have indica and japonica specificity ( figure 1 ); (2) The amplification effect of some markers is not good, and the band patterns are blurred; (3) Although the amplification products of these indica and japonica markers are not consistent, and the time of electrophoretic typing is different, there is no specific (4) After traditional PAGE gel electrophoresis, silver staining is used to identify the band pattern, and the operation process is cumbersome and time-consuming; (5) The identification of indica and japonica attributes is only based on the comparison with the control varieties 9311 and 9311. Nippon Clear’s comparison risks miscalculation

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