Structuring and preparation method of Ad5-M2m used as general influenza vaccine virus seed

A kind of influenza vaccine, ad5-m2m technology, applied in the field of vaccine preparation, can solve the problem that inactivated vaccines cannot cross-protect different branches of the same subtype, cannot induce cellular immune response and mucosal immune response, and the speed of vaccine update cannot keep up with the speed of virus mutation and other issues, to achieve the effect of great application value, low cost, and good immunogenicity
CN106480095AInactive Publication Date: 2017-03-08SOUTH CHINA AGRI UNIV +2
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2017-03-08
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention discloses a structuring and preparation method of Ad5-M2m used as general influenza vaccine virus seed; the Ad5-M2m recombinant adenovirus is prepared as a general influenza vaccine virus seed; the preparation method includes steps of structuring multiple flu subtype conservative immunogen M2m recombinant adenovirus and establishing the virus seed; inoculating and cultivating Ad5-M2m; harvesting Ad5-M2m; preliminary immunizing of Ad5-M2m, and so on. The Ad5-M2m used as general influenza vaccine virus seed prepared by the invention is wide in antigen spectrum, stable in quality, high in purity, good in safety, low in cost and others; the method is applicable to large-scale production and has big application value.
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Description

technical field

[0001] The invention belongs to the technical field of vaccine preparation, in particular to a method for constructing and preparing Ad5-M2m recombinant adenovirus as a universal influenza vaccine virus seed by using a genetic engineering method. Background technique

[0002] At present, influenza vaccines are mainly used in the prevention of influenza. The vaccine for avian influenza is mainly an inactivated whole virus vaccine. This vaccine is safe and well tolerated, and can induce a high humoral immune response, but cannot induce effective cellular immune response and mucosal immune response. Immune response, and the incidence of adverse reactions during the vaccination process is also high, which affects the egg production rate and meat quality of poultry; at the same time, due to the continuous variation and recombination of avian influenza viruses, it is common that inactivated vaccines cannot cross-protect different branches of the same subtype The ph...

Claims

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