Method of paraffin sectioning the leaves of Litectinus chinensis

Abstract

The invention discloses a paraffin slicing method for corydalis saxicola blades. The method comprises the following steps: fixing a sample, dehydrating, transparently processing, waxing, embedding, slicing and unfolding slices, de-waxing, dyeing and sealing slices. The dyeing step further comprises the following steps: before transparently processing, putting the dehydrated corydalis saxicola blades into a safranine solution in volume concentration of 0.8-1.2%, and dip-dyeing for 8-12h; rehydrating a slide carrying the corydalis saxicola blades in the dyeing step; after rehydrating, putting into an alcoholic solution for separating colors; adding acetic acid at the volume ratio of 1 / (800-1000) into the alcoholic solution used for separating colors; and finally, putting into a fast green solution in volume concentration of 0.8-1.2%, and dyeing for 5-15 seconds. The corydalis saxicola blades prepared according to the invention are clear in outline tissues and are suitable for identification of medicinal materials.

Description

technical field

[0001] The present invention relates to the field of histology. More specifically, the present invention relates to a method for paraffin sectioning of the leaves of Litectinum chinensis. Background technique

[0002] Corydalis saxicola Bunting (Corydalis saxicola Bunting) is a plant of the Papaveraceae family, a perennial herb, distributed in Guangxi, Guangdong, Fujian, Hunan and other provinces of China. The whole herb is used as medicine, and the whole herb contains active ingredients such as dehydrocarvitin (petidine), which has significant antibacterial, anti-inflammatory, analgesic and strong stabilizing effects, and it has been found to have the effect of inhibiting tumor cells. , hepatitis, liver cirrhosis, liver cancer and other diseases. Yanhuanglian is a famous Zhuang Yao medicine variety. The distribution of Yanhuanglian plants is limited to limestone mountainous areas, and it is a unique species in Shishan. Due to the poor habitat conditions, ...

Examples

Embodiment 1

[0031] (1) Fixing: After harvesting the leaves of fresh Litectin chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the vertical leaf midrib is cut across, and each piece is about 10mm long, wide 5mm, puts into the FAA fixative that volume concentration is prepared by 70% alcohol and fixes for 24 hours, FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde volume The ratio is 90:5:5.

[0032] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 10 minutes, and stayed for 45 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 85% a...

Embodiment 2

[0041] (1) Fixing: After harvesting the leaves of fresh Litectin chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the vertical blade midrib is cut across, and each piece is about 5mm long, wide 3mm, puts into the FAA fixative that volume concentration is prepared by 70% alcohol and fixes for 24 hours, and FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde volume The ratio is 90:5:5.

[0042] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 15 minutes, and stayed for 55 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 8...

Embodiment 3

[0051] (1) Fixing: After harvesting the leaves of fresh Litectin chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the vertical leaf midrib is cut across, and each piece is about 8mm long, wide 4mm, puts into the FAA fixative that volume concentration is prepared by 70% alcohol and fixes for 24 hours, and FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde volume The ratio is 90:5:5.

[0052] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuum for 15 minutes, and stay for 60 minutes; pour out the volume concentration of 80% alcohol, add the volume concentration of 85% al...

technical field

[0001] The present invention relates to the field of histology. More specifically, the present invention relates to a method for paraffin sectioning of the leaves of Litectinum chinensis. Background technique

[0002] Corydalis saxicola Bunting (Corydalis saxicola Bunting) is a plant of the Papaveraceae family, a perennial herb, distributed in Guangxi, Guangdong, Fujian, Hunan and other provinces of China. The whole herb is used as medicine, and the whole herb contains active ingredients such as dehydrocarvitin (petidine), which has significant antibacterial, anti-inflammatory, analgesic and strong stabilizing effects, and it has been found to have the effect of inhibiting tumor cells. , hepatitis, liver cirrhosis, liver cancer and other diseases. Yanhuanglian is a famous Zhuang Yao medicine variety. The distribution of Yanhuanglian plants is limited to limestone mountainous areas, and it is a unique species in Shishan. Due to the poor habitat conditions, ...

Examples

Embodiment 1

[0031] (1) Fixing: After harvesting the leaves of fresh Litectin chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the vertical leaf midrib is cut across, and each piece is about 10mm long, wide 5mm, puts into the FAA fixative that volume concentration is prepared by 70% alcohol and fixes for 24 hours, FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde volume The ratio is 90:5:5.

[0032] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 10 minutes, and stayed for 45 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 85% a...

Embodiment 2

[0041] (1) Fixing: After harvesting the leaves of fresh Litectin chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the vertical blade midrib is cut across, and each piece is about 5mm long, wide 3mm, puts into the FAA fixative that volume concentration is prepared by 70% alcohol and fixes for 24 hours, and FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde volume The ratio is 90:5:5.

[0042] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuumed for 15 minutes, and stayed for 55 minutes; poured out a volume concentration of 80% alcohol, added a volume concentration of 8...

Embodiment 3

[0051] (1) Fixing: After harvesting the leaves of fresh Litectin chinensis, rinse the floating dust on the surface with tap water, and absorb the water on the surface of the leaves. Then the vertical leaf midrib is cut across, and each piece is about 8mm long, wide 4mm, puts into the FAA fixative that volume concentration is prepared by 70% alcohol and fixes for 24 hours, and FAA fixative is 70% alcohol by volume concentration: glacial acetic acid: formaldehyde volume The ratio is 90:5:5.

[0052] (2) Dehydration: Take the material out of the FAA fixative solution, absorb the excess FAA fixative solution with filter paper, put it into a flat-bottomed glass tube with a rubber stopper, add the volume concentration to 80% alcohol, and the volume concentration is the amount of 80% alcohol added It is more than 20 times the volume of the material, vacuum for 15 minutes, and stay for 60 minutes; pour out the volume concentration of 80% alcohol, add the volume concentration of 85% al...

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