Method for Rapidly Obtaining Crude Extract of Chlorogenic Acid

A technology of chlorogenic acid and crude extract, which is applied in the field of rapid acquisition of chlorogenic acid crude extract, can solve problems such as insufficient development resources, achieve time-consuming and labor-intensive production, and reduce manpower and material resources

Active Publication Date: 2020-11-10
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to achieve the above object, the present invention provides a method for quickly obtaining the crude extract of chlorogenic acid, which can quickly extract the active ingredient of Usurivavir, and solves the problem of insufficient development resources

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  • Method for Rapidly Obtaining Crude Extract of Chlorogenic Acid

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0026] A method for rapidly obtaining the crude extract of chlorogenic acid, specifically according to the following steps:

[0027] Step 1, medium configuration and sterilization

[0028] The configuration of the medium: 1 / 2MS is used as the basic medium, and 6-BA 0.3mg·L is added -1 , 2,4-D 3mg·L -1 , IBA1mg·L -1 As a medium growth regulator, the medium agar content is 7.0g L -1 , the sucrose content is 30.0g·L -1 , add double distilled water to dissolv...

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Abstract

The invention discloses a method for rapidly obtaining a crude extract of chlorogenic acid. The method comprises the following steps: configuring and sterilizing a culture medium; disinfecting rhizome Lepisorus ussuriensis; inoculating an explant; culturing rhizome callus tissues; inducing the rhizome callus tissues; proliferating the rhizome callus tissues; and extracting effective ingredients from the callus tissues of Lepisorus ussuriensis. The method provided by the invention can rapidly extract active ingredients of Lepisorus ussuriensis and solves a problem of insufficient development resources.

Description

technical field [0001] The invention belongs to the technical field of preparation of anti-inflammatory drugs, and relates to a method for rapidly obtaining crude extract of chlorogenic acid. Background technique [0002] Lepisorus ussuriensis (Regel et Maack) Ching is a plant of the genus Lepisorus ussuriensis (Regel et Maack) Ching of the family Pteridaceae. In China, it is distributed in Anhui, Henan, Shandong, Hebei, Liaoning, Jilin, and Heilongjiang. According to data records, the main active ingredient of Ussurivavir is chlorogenic acid, which has the effects of clearing away heat and detoxification, diuresis, relieving cough, and stopping bleeding. It can be used to treat dysuria, dysuria, lung heat cough, asthma, sore throat, sore Swelling poison, rheumatism pain, knife wound bleeding. Ussuriwawe grows on mountain trunks or rocks at an altitude of about 800m, and the plants are relatively short (more than 10 cm high). At present, this plant is only used among folk...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C07C67/48C07C67/56C07C69/732A61K31/216A01H4/00A61P11/14A61P7/04A61P13/00A61P11/06A61P19/06
CPCA01H4/001A01H4/008C07C67/48C07C67/56C07C69/732
Inventor王丽红费秀斌吴莉莉黄佳滨马显华姜晓婷
OwnerJIAMUSI UNIVERSITY