Primer pair, kit and method for identifying storage flour beetles

A primer pair and kit technology, applied in the field of molecular biology, to achieve the effects of strong specificity, repeatable operation, and simple operation

Active Publication Date: 2017-05-24
HENAN UNIVERSITY OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no biological product for identifying or assisting in the identification of powder thieves on the market at present.

Method used

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  • Primer pair, kit and method for identifying storage flour beetles
  • Primer pair, kit and method for identifying storage flour beetles
  • Primer pair, kit and method for identifying storage flour beetles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 is aimed at the design and synthesis of the specific primers of 3 kinds of storage powder robbers

[0043] 1.1 Acquisition of COI sequences of 3 kinds of warehouse fans

[0044] Experimental materials: Involving 3 kinds of storage powder pirates, specifically small powder pirates, Ji powder pirates, and sub-flat powder pirates; among them, small powder pirates involve the following geographical populations: Fujian Xiamen, Fujian Zhangzhou; Ji powder pirates involve the following geographical populations: Fujian Longyan, Liuzhou in Guangxi, Jiangmen in Guangdong, Shanwei in Guangdong, Zhongshan in Guangdong, and Shenzhen; the sub-flat fleas are related to the following geographical populations: Zhaoqing in Guangdong, Longyan in Fujian, Wenzhou in Zhejiang, Jiangmen in Guangdong, and Zhongshan in Guangdong.

[0045] 1.1.1 Extraction of Genomic DNA

[0046] Ezup Column Animal Genomic DNA Extraction Kit (Shenggong, Shanghai) was used to extract DNA, and some m...

Embodiment 2

[0070] Example 2 Utilizes specific primers for the COI sequences of 3 kinds of storage powder robbers to carry out specific detection

[0071] 2.1 Experimental materials

[0072] Three types of warehouse fans are involved, specifically, small fans, Ji fans, and sub-flat fans. Among them, Xiaofen Pirates involve the following geographical populations: Xiamen, Fujian, Zhangzhou, Fujian; Jifen Pirates involve the following geographical populations: Fujian Longyan, Guangxi Liuzhou, Guangdong Jiangmen, Guangdong Shanwei, Guangdong Zhongshan, Shenzhen; sub-flat Fen Pirates involve the following geographical populations: Zhaoqing, Guangdong, Longyan, Fujian, Wenzhou, Zhejiang, Jiangmen, Guangdong, Zhongshan, Guangdong.

[0073] 2.2 Experimental method

[0074] The Ezup column type animal genome DNA extraction kit was used to extract DNA (Shenggong, Shanghai) to extract the total DNA of the whole body of a single adult beetle to be tested. Carried out with reference to the kit inst...

Embodiment 3

[0085] Example 3 Sensitivity detection of 3 kinds of storage powder thief COI sequence-specific primers

[0086] 3.1 Experimental materials

[0087] Three types of warehouse fans are involved, specifically, small fans, Ji fans, and sub-flat fans. Among them, the geographical populations of Xiaofen Pirates are: Xiamen, Fujian; Zhangzhou, Fujian; the geographical populations of Jifen Pirates are: Longyan, Fujian; Liuzhou, Guangxi; Jiangmen, Guangdong; Shanwei, Guangdong; Zhongshan, Guangdong; Shenzhen; The geographical populations of Fenbo are: Zhaoqing, Guangdong; Longyan, Fujian; Wenzhou, Zhejiang; Jiangmen, Guangdong; Zhongshan, Guangdong.

[0088] 3.2 Experimental method

[0089] The Ezup column type animal genome DNA extraction kit was used to extract DNA (Shenggong, Shanghai) to extract the total DNA of the whole body of a single adult beetle to be tested. For specific operations, refer to the kit instructions. With the obtained genomic DNA as a template, each primer p...

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Abstract

The invention belongs to the technical field of molecular biologics, in particular relates to a primer pair and a kit for identification or auxiliary identification on storage flour beetles, and further relates to a method for identification of the storage flour beetles. The primer pair for identification on the storage flour beetles is any one of primer pairs as follows: a primer pair consisting of sequences of SEQ ID No:1 and SEQ ID No:2 as shown in the specification, a primer pair consisting of sequences of SEQ ID No:3 and SEQ ID No:4 as shown in the specification, and a primer pair consisting of sequences of SEQ ID No:5 and SEQ ID No:6 as shown in the specification. The kit provided by the invention comprises at least one of the primer pairs. By adopting the primer pairs for identification on the storage flour beetles, and the kit comprising the prime pair, and the method, three types of storage flour beetles can be identified, specificity is good, operation is simple and repeatable, sensitivity is high and relatively stable, and ideal methods are provided for identifying the storage flour beetles.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a primer pair and a kit for identifying or assisting the identification of storage powder pirates, and a method for identifying or assisting the identification of storage powder robbers. Background technique [0002] Flour beetles, belonging to Coleptera, Tenebrionidae, and Palorus, are common pests in the protection of stored grains in the world. This genus of insects is widely distributed in the world. At present, there are 7 species of stored-grain pests of the genus Fenus in the world, and there are 3 species in total in our country, namely the small Fenba, Ji Fen, and sub-flat Fen. [0003] Mesopodia, an economically important species of Pterocaridae insects, is a secondary stored grain pest. It mainly harms flour, wheat, corn, rice, and their processed products. At the same time, these pests will also produce some excrement waste, which will endange...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888
Inventor 鲁玉杰赵亚茹王争艳彭华刘凤杰苗世远张晨光
Owner HENAN UNIVERSITY OF TECHNOLOGY
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