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Bacillus amyloliquefaciens strain antagonistic to lycium chinensis root rot and application thereof

A technology of amyloliquefaciens and Lycium barbarum root, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of no Bacillus amyloliquefaciens, etc., and achieve the effects of improving growth, increasing yield, and inhibiting outbreaks

Active Publication Date: 2017-05-31
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, in agricultural applications, although there have been reports that Bacillus amyloliquefaciens has activity against a variety of pathogenic fungi, so far, there are few reports on the use of Bacillus amyloliquefaciens to make biocontrol agents for the prevention and treatment of plant diseases.
However, there is no report of Bacillus amyloliquefaciens for the prevention and treatment of Lycium barbarum root rot

Method used

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  • Bacillus amyloliquefaciens strain antagonistic to lycium chinensis root rot and application thereof
  • Bacillus amyloliquefaciens strain antagonistic to lycium chinensis root rot and application thereof
  • Bacillus amyloliquefaciens strain antagonistic to lycium chinensis root rot and application thereof

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Experimental program
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Effect test

Embodiment 1

[0029] Example 1: Isolation and identification of bacterial strains

[0030] 1. Isolation and screening of strains:

[0031] (1) Separation of strains

[0032] The rhizosphere soil samples of Lycium barbarum were collected from Shilaba Village, Zhongning County, Ningxia Province. Weigh 10.0 grams of Lycium barbarum rhizosphere soil, add 90 mL of sterile water for every 10 grams of soil, put it into a pre-prepared triangular flask with glass beads, place it on a 37°C constant temperature shaker, and shake at 180r / min for 20-30min . Gradient dilution method, the soil suspension was diluted to 10 -2 、10 -3 、10 -4 、10 -5 、10 -6different gradients. Draw 0.1 mL of soil solution with appropriate dilution and place it on the LB plate medium, and spread it evenly (in this experiment, 10 -4 、10 -5 、10 -6 Three gradients were applied). Cultivate in a constant temperature incubator at 37°C for 24 hours, and pick a single colony. The obtained strains were purified by the three...

Embodiment 2

[0050] Embodiment 2: the preparation of bacterial strain GQJK49 biocontrol agent

[0051] The specific preparation method is as follows:

[0052] The Bacillus amyloliquefaciens GQJK49 that was preserved on the slant to antagonize wolfberry root rot was transferred to a test tube containing 5 mL of bean sprout juice liquid medium, and cultured in a constant temperature shaker at 37°C and 180 rpm for 12 hours. Then inoculate into 100mL / 250mL liquid bean sprouts juice culture medium according to 1% inoculum amount, put in 37 ℃, 180rpm constant temperature shaker and cultivate 24h, make concentration be 3 * 10 8 cfu / mL bacterial suspension.

Embodiment 3

[0053] Embodiment 3: field test

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Abstract

The invention discloses a bacillus amyloliquefaciens strain antagonistic to lycium chinensis root rot. The bacillus amyloliquefaciens strain is classified and named after bacillus amyloliquefaciens GQJK49, and is preserved on January 10, 2016 in the China General Microbiological Culture Collection Center, called as CGMCC for short and located in the Institute of Microbiology, Chinese Academy of Sciences at Number 3, Yard 1, Western Beichen Road, Chaoyang District, Beijing, with the strain preservation number of CGMCC NO. 13255. The bacillus amyloliquefaciens GQJK49 obtained by separation has a relatively good antagonistic effect on lycium chinensis root rot, a novel biocontrol bacterium is provided for biological prevention and control of lycium chinensis root rot, and the types of biocontrol preparations are increased.

Description

technical field [0001] The invention relates to a strain of bacillus amyloliquefaciens antagonizing wolfberry root rot and application thereof, belonging to the field of biotechnology. Background technique [0002] Lycium barbarum is a famous and precious traditional Chinese medicine. Lycium barbarum fruit has high nutritional, health care and medicinal value. It is also known as "four treasures of traditional Chinese medicine" together with Qiongzhen Ganoderma lucidum, Changbai Mountain ginseng and Dong'e Ejiao. With the development of the economy and the progress of the society, people are more and more aware of the value of wolfberry, prompting people to carry out extensive product development of wolfberry, and achieved important results. Ningxia wolfberry has the largest cultivation area in China, mainly distributed in Northwest China. In recent years, with the continuous expansion of wolfberry planting area, the incidence of wolfberry root rot in old production areas a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 杜秉海丁延芹刘凯汪城墙姚良同马锦锦
Owner SHANDONG AGRICULTURAL UNIVERSITY
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